Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

13.8K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
13.8K
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

4.6K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
4.6K
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

3.9K
3.9K
Membrane Fluidity01:26

Membrane Fluidity

12.2K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
12.2K
Intrinsically Disordered Proteins02:18

Intrinsically Disordered Proteins

18.3K
Intrinsically disordered proteins are a group of proteins that do not fold into specific three-dimensional structures. Their structural flexibility allows them to complement ordered proteins to perform functions that are inaccessible to rigid structures. They are more common in eukaryotes than prokaryotes and may either be exclusively intrinsically disordered or hybrid proteins, consisting of a mix of ordered and disordered regions. The absence of a rigid structure in these proteins can be...
18.3K
Fluid Mosaic Model01:19

Fluid Mosaic Model

13.2K
Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich...
13.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Heat treatment influences adsorption of potato protein at the oil-water interface and emulsion droplet stability at short timescales.

Journal of colloid and interface science·2026
Same author

Silk Fibroin Aggregates at the Air-Water Interface: Amyloid-like Fibrils vs. Self-Assembled Networks.

International journal of molecular sciences·2026
Same author

Structure-functionality impact of heat treatment on the interfacial adsorption of whey proteins at millisecond time scales.

Journal of colloid and interface science·2026
Same author

Size- and density-dependent gastric emptying of emulsion-alginate beads for tailored <i>in vitro</i> intestinal lipolysis.

Current research in food science·2026
Same author

Unifying lipolysis under intestinal INFOGEST conditions by total surface area and coalescence.

Food & function·2025
Same author

Engineered materials for sustainable development in environmental and healthcare applications.

Advances in colloid and interface science·2025

Related Experiment Video

Updated: Sep 24, 2025

Author Spotlight: Characterization of Low-Affinity Protein Interactions in Solution Using MassFluidix Technology
06:39

Author Spotlight: Characterization of Low-Affinity Protein Interactions in Solution Using MassFluidix Technology

Published on: January 26, 2024

2.4K

Interfacial protein-protein displacement at fluid interfaces.

Emma B A Hinderink1, Marcel B J Meinders2, Reinhard Miller3

  • 1Laboratory of Food Process Engineering, Wageningen University and Research, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands; TiFN, P.O. Box 557, 6700 AN Wageningen, the Netherlands.

Advances in Colloid and Interface Science
|May 9, 2022
PubMed
Summary

Protein blends stabilize emulsions, but adsorbed proteins can be displaced over time, potentially destabilizing the product. This review covers methods and models to study these interfacial dynamics.

Keywords:
Adsorption modelBrownian dynamics simulationsCompetitive adsorptionInterfacial rheologyProtein-stabilised emulsionSurface equation of state

More Related Videos

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
04:45

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

Published on: February 10, 2022

7.2K
In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
07:42

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

Published on: March 19, 2010

10.9K

Related Experiment Videos

Last Updated: Sep 24, 2025

Author Spotlight: Characterization of Low-Affinity Protein Interactions in Solution Using MassFluidix Technology
06:39

Author Spotlight: Characterization of Low-Affinity Protein Interactions in Solution Using MassFluidix Technology

Published on: January 26, 2024

2.4K
Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
04:45

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

Published on: February 10, 2022

7.2K
In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
07:42

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

Published on: March 19, 2010

10.9K

Area of Science:

  • Food science and technology
  • Colloid and surface chemistry
  • Materials science

Background:

  • Protein blends are crucial for stabilizing emulsions, forming complex, non-equilibrated interfacial structures.
  • The initial interfacial composition depends on competitive protein adsorption, which can change over time.
  • Rearrangements and displacement of adsorbed proteins can compromise interfacial film integrity, leading to emulsion destabilization.

Purpose of the Study:

  • To critically review experimental techniques for assessing interfacial composition, properties, and protein displacement mechanisms in protein blend-stabilized emulsions.
  • To evaluate the suitability of different techniques for studying protein displacement dynamics.
  • To discuss mechanistic physical models and simulation approaches for understanding interfacial behavior in complex mixed systems.

Main Methods:

  • Review of experimental techniques (e.g., interfacial rheology, spectroscopy) for characterizing emulsion interfaces.
  • Analysis of comparative studies between model interfaces and real emulsions.
  • Discussion of physical models and Brownian dynamic simulations for interfacial displacement.

Main Results:

  • Various techniques provide different insights into interfacial composition, properties, and displacement mechanisms.
  • Comparative studies highlight the impact of minor components and fluid dynamics on interface formation.
  • Mechanistic models and simulations offer a framework for understanding protein interactions and displacement dynamics.

Conclusions:

  • Understanding protein displacement dynamics is critical for ensuring the long-term stability of protein-stabilized emulsions.
  • A combination of experimental and modeling approaches is necessary for comprehensive characterization of interfacial properties over time.
  • This review provides a valuable resource for researchers and industry professionals working with protein blend-stabilized emulsion systems.