Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An image analysis protocol using CellProfiler for automated quantification of post-ischemic cardiac parameters.

Alexander O Ward1,2, Vaibhao Janbandhu1,2, Gavin Chapman1,2

  • 1Victor Chang Cardiac Research Institute, Darlinghurst, NSW, Australia.

STAR Protocols
|May 10, 2022
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preemptive cardioprotection with a small molecule in rodents that suppresses genes predictive of heart failure.

Science advances·2026
Same author

Increased Risk of Disabilities in Children and Adolescents With Congenital Heart Disease of Any Severity.

Journal of the American Heart Association·2026
Same author

A three generation family with VACTERL association is found to have a rare form of diamond-blackfan anaemia.

European journal of human genetics : EJHG·2026
Same author

Prenatal and Maternal Contributors to Disease Severity in Congenital Heart Disease.

Maternal and child health journal·2026
Same author

Correction: Development of a robust induced pluripotent stem cell atrial cardiomyocyte differentiation protocol to model atrial arrhythmia.

Stem cell research & therapy·2026
Same author

Timing of NAD Deficiency During Organogenesis Dictates Defect Type and Penetrance.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026

This study introduces an automated CellProfiler pipeline to quantify cardiac remodeling after ischemia. The method reduces variability in analyzing complex scar tissue, improving high-throughput analysis of microscopy images.

Area of Science:

  • Cardiovascular Biology
  • Image Analysis
  • Computational Pathology

Background:

  • Assessing post-ischemic cardiac remodeling is challenging due to scar complexity and heterogeneity.
  • Manual quantification of cardiac tissue introduces inter-observer variability.
  • Automated image analysis offers an unbiased approach for high-throughput studies.

Purpose of the Study:

  • To develop and validate a CellProfiler-based pipeline for automated quantification of cardiac parameters post-ischemia.
  • To enable high-throughput analysis of confocal microscopy images.
  • To provide an adaptable protocol for other tissue types.

Main Methods:

  • Image preprocessing of confocal microscopy data.
  • Development of a CellProfiler pipeline for automated quantification.
Keywords:
Cell BiologyComputer sciencesMicroscopy

Related Experiment Videos

  • Analysis of capillary rarefaction, immune cell infiltration, cell death, and fibroblast proliferation.

    • Main Results:

    • Successful implementation of a CellProfiler pipeline for quantitative assessment of cardiac remodeling.
    • Demonstrated high-throughput analysis capabilities for post-ischemic cardiac parameters.
    • Quantification of key features including capillary rarefaction and immune cell infiltration.

    Conclusions:

    • The developed CellProfiler pipeline provides an unbiased and efficient method for quantifying post-ischemic cardiac remodeling.
    • This automated approach can reduce inter-observer variability in scar tissue analysis.
    • The protocol is adaptable for analyzing various tissue types in microscopy studies.