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Updated: Sep 24, 2025
Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
Published on: September 13, 2018
Protocols for measuring phosphorylation, subcellular localization, and kinase activity of Hippo pathway components
Min Luo1, Yuan Hu2, Xiawei Wei1
1Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy, National Clinical Research Center for Geriatrics, Cancer Center, West China Hospital, Sichuan University, No. 17, South of Renmin Road, Chengdu 610041, China.
Abstract:
The Hippo pathway plays critical roles in cell growth, differentiation, development, tissue homeostasis, and cancer. Here, we describe protocols to measure phosphorylation, subcellular localization, and kinase activity for core Hippo pathway components YAP and LATS. The phos-tag technology is particularly useful to quantitatively detect protein phosphorylation even without a phosphospecific antibody. We use HEK293A cells as an example, but the protocols can be applied to other cell lines and tissues. For complete details on the use and execution of this profile, please refer to Luo et al. (2020).
