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Updated: Sep 24, 2025

Monitoring Acupuncture Effects on Human Brain by fMRI
Published on: April 8, 2010
In vivo neuronal and astrocytic activation in somatosensory cortex by acupuncture stimuli
Xiao-Yue Chang1, Kai Chen2, Tong Cheng2
1Key Laboratory of CNS Regeneration (Minstry of Education), Guangdong-Hong Kong-Macau Institute of CNS Regeneration, Jinan University, Guangzhou, Guangdong Province; Department of Electrical and Electronic Engineering, The University of Hong Kong, Hong Kong Special Administrative Region, China.
Abstract:
Acupuncture is a medical treatment that has been widely practiced in China for over 3000 years, yet the neural mechanisms of acupuncture are not fully understood. We hypothesized that neurons and astrocytes act independently and synergistically under acupuncture stimulation. To investigate this, we used two-photon in vivo calcium recording to observe the effects of acupuncture stimulation at ST36 (Zusanli) in mice. Acupuncture stimulation in peripheral acupoints potentiated calcium signals of pyramidal neurons and astrocytes in the somatosensory cortex and resulted in late-onset calcium transients in astrocytes. Chemogenetic inhibition of neurons augmented the astrocytic activity. These findings suggest that acupuncture activates neuronal and astrocytic activity in the somatosensory cortex and provide evidence for the involvement of both neurons and astrocytes in acupuncture treatment.
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