Related Experiment Video

Updated: Sep 24, 2025

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
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In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

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miR-30a Serves as a Tumor Suppressor for Hepatocellular Carcinoma by Downregulating ADAMTS14

He-Ming Wang, Lai-Lin Fu, Yan-Xin Cui

    Clinical Laboratory
    |May 10, 2022
    PubMed
    Abstract

    Insights

    MicroRNA-30a (miR-30a) functions as a tumor suppressor in hepatocellular carcinoma (HCC) progression. This microRNA inhibits tumor growth and can serve as a biomarker for early HCC detection.

    Area of Science:

    • Oncology
    • Molecular Biology
    • Biochemistry

    Background:

    • Hepatocellular carcinoma (HCC) is a prevalent malignancy with limited therapeutic advancements for advanced stages.
    • The role of microRNA-30a (miR-30a) in HCC tumorigenesis requires further investigation.

    Purpose of the Study:

    • To elucidate the function of miR-30a in hepatocellular carcinoma (HCC) development.
    • To determine the relationship between miR-30a and ADAMTS14 in HCC.

    Main Methods:

    • Assessed miR-30a and ADAMTS14 expression in HCC tissues.
    • Utilized luciferase reporter assays to confirm the miR-30a-ADAMTS14 interaction.
    • Evaluated cell viability, apoptosis, migration, and invasion using MTT, flow cytometry, and Transwell assays.
    • Determined protein levels of ADAMTS14, β-catenin, and GSK-3β via Western blotting.

    Main Results:

    • miR-30a expression was inversely correlated with ADAMTS14 levels in HCC.
    • ADAMTS14 was identified as a direct target of miR-30a.
    • miR-30a overexpression suppressed ADAMTS14, cell viability, and apoptosis, while promoting apoptosis.
    • miR-30a inhibited GSK-3β and β-catenin phosphorylation, impacting the Wnt/β-catenin pathway.

    Conclusions:

    • miR-30a acts as a tumor suppressor in HCC progression.
    • miR-30a demonstrates potential as a biomarker for early prediction and diagnosis of HCC.

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