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Updated: Sep 24, 2025

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
Published on: June 27, 2022
An ESIPT-based fluorescent turn-on probe with isothiocyanate for detecting hydrogen sulfide in environmental and
Jae Kyong Kim1, So Yeon Bong1, Rackhyun Park2
1Department of Chemistry, Yonsei University, Wonju 26493, Republic of Korea.
Abstract:
A probe with an isothiocyanate group was synthesized and evaluated for its H2S sensing ability. Upon addition of H2S, the probe exhibited ratiometric properties during absorption with a red-shift. The probe exhibited fluorescent off-on responses towards H2S via the ESIPT process, due to the conversion of isocyanate into amine. UV-vis, fluorescence, and 1H NMR spectroscopic analyses were performed to investigate the sensing mechanism. The probe has a large Stokes shift, short response time, and low detection limit. It can be used to estimate H2S levels within the range of 0-36 nM. The practical applicability of the probe was demonstrated using water samples and living cells.
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