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Updated: Sep 23, 2025

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
Published on: May 2, 2018
Amplicon sequencing data profiling of bacterial community connected with the rhizospheric soil from sunflower plants
Olubukola Oluranti Babalola1, Blessing Chidinma Nwachukwu1, Ayansina Segun Ayangbenro1
1Food Security and Safety Focus Area, Faculty of Natural and Agricultural Sciences, North-West University, Private Mail Bag X2046, South Africa.
Abstract:
This article presents dataset on the bacterial community structure associated with rhizospheric sunflower soils from Lichtenburg, South Africa. The Illumina Miseq sequencing of 16S rRNA gene amplicon unveiled the bacterial community diversities as well as generated metagenomic library from sunflower rhizospheric soils to understand the diversities and distribution. The metagenome contained a total of 41,471 sequences with 45,975 operational taxonomic units (OTUs). Metagenome sequence information is obtainable at NCBI under the Bioproject assigned accession number PRJNA782103. Taxonomic hits distribution from USEARCH analysis at phylum level classification of BN_E discovered predominantly Actinobacteria (33.89%), followed by Proteobacteria (35.45%), Firmicutes (6.45%), Planctomycetes (4.12%), Chloroflexi (4.28%) and Gemmatimonadetes (2.40%). Also, USEARCH assisted analysis of BN_F sample also detected the prevalence of Actinobacteria (45.92%), Proteobacteria (23.23%), Firmicutes (3.84%), Planctomycetes (6.46%), Chloroflexi (4.94%) and Gemmatimonadetes (1.99%), which take part in vital ecological functions and biogeochemical activities needed for plant growth and health.

