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Author Spotlight: Understanding Microbe Adaptation Using Innovative Techniques for Exploring Thermophilic Evolution
Published on: June 14, 2024
Adaptations of Bacillus shacheensis HNA-14 required for long-term survival under osmotic challenge: a multi-omics
Xiufeng Long1, Jiewei Tian1, Xuepin Liao1,2
1Department of Biomass and Leather Engineering, Sichuan University Chengdu 610065 PR China xpliao@scu.edu.cn yqtian@scu.edu.cn.
Abstract:
Genomic sequence, transcriptomic, metabolomic and fatty acid analyses of strain HNA-14 were performed to understand the mechanism of salt tolerance for long-term survival. The results indicated that strain HNA-14 has different osmotic resistance mechanisms for long-term survival and short-term salt stress. The cells mainly synthesized compatible solutes to resist osmotic pressure when cultured under nutrient deficient conditions, while they can slow down the synthesis rate and uptake from the environment when cultured under a nutritionally rich environment. Also, the amounts of branched and unsaturated fatty acids in the cell membrane are maintained to a high degree (>50%) to maintain the fluidity of the cell membrane; when the cells are cultured in a high osmotic environment for long-term survival, they may increase the content of branched fatty acids and phosphoric fatty acids to increase the fluidity of the cell membrane to resist the high osmotic pressure.
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