pH-responsive polymeric vesicles from branched copolymers
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Updated: Sep 23, 2025

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
Published on: March 1, 2013
Jinglun Zhou1,2, Linlin Li3, Weishan Wang3
1Key Laboratory of Special Functional Aggregated Materials, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University Jinan 250100 China fsy@sdu.edu.cn.
Abstract:
A new type of branched copolymer, poly(l-lactide)2-b-poly(l-glutamic acid) (PLLA2-PLGA), based on polypeptide PLGA is synthesized by the ring-opening polymerization (ROP) of N-carboxyanhydride of γ-benzyl-l-glutamate (BLG-NCA) with amino-terminated PLLA2-NH2 and subsequent deprotection. The branched copolymer is characterized by 1H NMR, FTIR and GPC measurements. The self-assembly of the copolymers in aqueous media has been systematically discussed. A pyrene probe has been used to demonstrate the aggregated formation of PLLA2-PLGA in solution by measuring the critical micelle concentration (cmc). The morphology and size of the micelles have further been studied by transmission electron microscopy (TEM), dynamic light scattering (DLS) and field emission scanning electron microscopy (ESEM). We demonstrated that the Rh of the vesicle is depending on solution pH and salt concentration. The vesicles show good stability with remained shapes and sizes during the lyophilizing process. These vesicles have great potential in the application of drug delivery.
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