Complement activation by gold nanoparticles passivated with polyelectrolyte ligands

Gold nanoparticles passivated by polyelectrolyte ligands are widely used to confer stability and biofunctionality. While nanoparticles and polyelectrolytes have been reported as activators, their ability to activate the complement system as hybrid polyelectrolyte-coated nanoparticles is poorly characterized. Here, we found that gold nanoparticles passivated by common polyelectrolytes activated the system differently. The surface area of AuNPs appeared to be a major determinant of complement activation level as it determined the amount of adsorbed polyelectrolytes. Although a moderate negative correlation between AuNP surface hydrophilicity and their activation level was observed, the surface charge and functional group of polyelectrolyte ligands also influenced the final complement activation level.