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Related Experiment Video

Updated: Sep 23, 2025

Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System

Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System

Published on: February 11, 2022

Open-Ended Inquiry into Zebrafish Nerve Development Using Image Analysis.

Sarah C Petersen¹

¹Department of Neuroscience and Department of Biology, Kenyon College, Gambier, OH 43022.

Journal of Undergraduate Neuroscience Education : JUNE : a Publication of FUN, Faculty for Undergraduate Neuroscience

|May 11, 2022

Summary

This summary is machine-generated.

Remote undergraduate students can conduct authentic scientific research using zebrafish neural development image analysis. This approach enhances learning and retention in introductory biology courses, even with limited lab access.

Keywords:

Fiji ImageJ authentic inquiry first-year research image analysis lateral line motor nerves neuroanatomy neurodevelopment open-ended laboratory remote learning sensory nerves zebrafish

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Area of Science:

Neuroscience
Developmental Biology
Undergraduate Education

Background:

Open-ended lab projects boost science student success and retention.
Remote learning presents challenges for organismal research projects.
Zebrafish offer a model for studying vertebrate development due to rapid, external embryonic development.

Purpose of the Study:

To describe a method for remote undergraduate students to conduct authentic research using zebrafish neural development.
To enable students to develop and test biological questions using image analysis.
To enhance skills in organismal observation, data collection, and scientific communication for remote learners.

Main Methods:

Utilized image analysis of zebrafish neural development for an introductory biology lab course.
Students analyzed provided images comparing zebrafish mutants and wild-type siblings.
Developed and applied techniques to analyze neuron and glia position, organization, and morphology.

Main Results:

Remote first-year students independently formulated and tested biological questions.
Students mastered image analysis techniques for zebrafish neural development.
Students successfully communicated their findings in journal article and oral presentation formats.

Conclusions:

Image analysis of zebrafish neural development provides an accessible platform for remote undergraduate research.
This module effectively teaches neural development, data analysis, and scientific communication principles.
The approach is adaptable for both on-campus and remote students, fostering engagement and skill development.