An amperometric glucose biosensor based on PEDOT nanofibers

Area of Science:

• Materials Science
• Nanotechnology
• Electrochemistry

Background:

• Development of cost-effective and efficient biosensors is crucial for disease diagnosis.
• Conducting polymer nanofibers offer unique properties for biosensor applications.
• Poly(3,4-ethylenedioxythiophene) (PEDOT) is a promising material for electrochemical sensors.

Purpose of the Study:

• To present a simple, low-cost approach for producing PEDOT nanofiber biosensors.
• To characterize the fabricated PEDOT nanofibers and their performance in glucose detection.
• To evaluate the sensitivity, stability, and selectivity of the developed biosensors.

Main Methods:

• PEDOT nanofibers were synthesized via chemical vapor polymerization of EDOT on electrospun PAN mats.
• Glucose oxidase (GOx) was immobilized onto the PEDOT nanofibers to create biosensors.
• Biosensor performance was evaluated by measuring current response against glucose concentration under different operating conditions.

Main Results:

• Homogeneous, porous, and electroactive PEDOT nanofibers were successfully fabricated.
• The PEDOT-based biosensors demonstrated good sensitivity, selectivity, and stability.
• One biosensor configuration showed a sensitivity of 272.58 μA mM⁻¹ cm⁻² and another exhibited a low limit of detection (LOD) of 2.9 μM.

Conclusions:

• The developed PEDOT nanofiber biosensors are simple to construct, cost-effective, and exhibit excellent performance characteristics.
• These interference-free biosensors demonstrate significant potential for commercialization in glucose monitoring applications.
• The study highlights the viability of PEDOT nanofibers as a platform for advanced biosensor development.