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Sensitive detection of ascorbic acid using screen-printed electrodes modified by electroactive melanin-like

Wicem Argoubi1, Amal Rabti1, Sami Ben Aoun2

  • 1Tunis El Manar University, Chemistry Department, Laboratory of Analytical Chemistry and Electrochemistry (LR99ES15) Campus Universitaire de Tunis El Manar 2092 Tunis Tunisia noureddine.raouafi@fst.utm.tn.

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This study introduces a novel, enzyme-less electrochemical sensor for detecting ascorbic acid (AA) using melanin-like nanoparticles. The developed sensor offers high sensitivity and selectivity for AA detection in biological samples.

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Area of Science:

  • Electrochemistry
  • Nanomaterials Science
  • Analytical Chemistry

Background:

  • Ascorbic acid (AA) detection is crucial in biological and clinical diagnostics.
  • Existing methods often rely on enzymes, which can be unstable or costly.
  • Development of enzyme-less, sensitive, and selective electrochemical sensors is highly desirable.

Purpose of the Study:

  • To design and characterize an enzyme-less electrochemical electrode for sensitive and selective ascorbic acid detection.
  • To investigate the electrocatalytic properties of melanin-like nanoparticles (Mel-NPs) for ascorbic acid sensing.
  • To evaluate the performance of the modified electrode in real biological samples.

Main Methods:

  • Modification of a screen-printed electrode with melanin-like nanoparticles (Mel-NPs).
  • Electrochemical characterization using cyclic voltammetry.
  • Determination of dynamic window, detection limit, and quantification limit.
  • Validation of the sensor's performance in human blood serum, urine, and saliva samples.

Main Results:

  • The melanin-modified electrode demonstrated high electrocatalytic activity towards ascorbic acid.
  • Mel-NPs acted as effective electron shuttles, enhancing signal transduction.
  • The sensor exhibited a wide dynamic window (5–500 ppb) with low detection (0.07 ppb) and quantification (0.23 ppb) limits.
  • Successful determination of ascorbic acid in biological samples with satisfactory recovery.

Conclusions:

  • The developed enzyme-less electrochemical sensor based on Mel-NPs offers a promising platform for accurate ascorbic acid detection.
  • The sensor's high sensitivity, selectivity, and applicability to real samples make it suitable for various diagnostic applications.
  • This approach provides an efficient and cost-effective alternative to traditional enzyme-based detection methods.