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Updated: Jun 29, 2026

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
Published on: May 29, 2011
A simple two-photon turn-on fluorescent probe for the selective detection of cysteine based on a dual PeT/ICT
Xiani Chen1, Hang Xu1, Shengnan Ma1
1Shanghai Key Laboratory of New Drug Design, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, State Key Laboratory of Bioengineering Reactor, East China University of Science and Technology 130 Meilong Road Shanghai 200237 China kylou@ecust.edu.cn.
Abstract:
Herein, a simple two-photon turn-on fluorescent probe, N-(6-acyl-2-naphthayl)-maleimide (1), based on a dual PeT/ICT quenching mechanism is reported for the highly sensitive and selective detection of cysteine (Cys) over other biothiols. The probe was applied in the two-photon imaging of Cys in cultured HeLa cells, excited by a near-infrared laser at 690 nm.
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