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Related Experiment Video

Updated: Sep 23, 2025

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
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Micro-PCR chip-based multifunctional ultrafast SARS-CoV-2 detection platform.

Hao Yin1,2, Zhaoduo Tong1,2, Chuanjie Shen1,2

  • 1State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China. wuzhx@mail.sim.ac.cn.

Lab on a Chip
|May 11, 2022
PubMed
Summary

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A new rapid PCR platform enables low-cost, simultaneous screening and accurate quantification of infectious pathogens like SARS-CoV-2. This versatile tool offers fast, reliable point-of-care diagnosis for infectious diseases within 15 minutes.

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Medical Diagnostics

Background:

  • Effective infectious disease management requires low-cost, rapid, and accurate point-of-care screening and diagnostic tools.
  • Current diagnostic methods may not always meet the demands for speed and simultaneous analysis in point-of-care settings.

Purpose of the Study:

  • To develop and validate a multifunctional rapid PCR platform for simultaneous screening, identification, and quantification of viruses.
  • To assess the platform's performance for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection and viral load quantification.

Main Methods:

  • Development of a rapid PCR platform integrating simultaneous screening and precise quantification capabilities.
  • Utilizing a droplet microfluidic chip for high-throughput nucleic acid assays across a wide concentration range.

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  • Testing the platform with clinical samples for SARS-CoV-2 detection and viral load determination.

    • Main Results:

    • The platform achieved a detection limit of 10 nucleic acid copies/test within 15 minutes for 9 samples.
    • Assays across four orders of magnitude of concentration were completed in under 15 minutes.
    • Clinical sample analysis demonstrated accurate differentiation of positive/negative cases and consistent viral load quantification compared to standard RT-qPCR.

    Conclusions:

    • The developed rapid PCR platform is a promising, versatile tool for point-of-care diagnosis of infectious diseases.
    • It offers a fast, accurate, and cost-effective solution for simultaneous screening and quantification of viral pathogens.
    • The platform's capabilities support efficient infectious disease management in point-of-care settings.