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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Chimeric Peptide-Based Biomolecular Constructs for Versatile Nucleic Acid Biosensing.

Richard V Lee¹, Hadi M Zareie¹, Mehmet Sarikaya¹

¹GEMSEC, Genetically Engineered Materials Science and Engineering Center, Department of Materials Science and Engineering, University of Washington, Seattle, Washington 98195, United States.

ACS Applied Materials & Interfaces

|May 11, 2022

Summary

This summary is machine-generated.

Researchers developed novel bifunctional biomolecules for disease detection. These constructs immobilize nucleic acid probes onto sensor surfaces, enabling sensitive DNA detection with improved target capture efficacy.

Keywords:

DNA genetically engineered peptides for inorganics (GEPI)modular chimeric peptide nucleic acid biosensing peptide nucleic acid (PNA)solid-binding peptide (SBP)surface functionalization

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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

Area of Science:

Biomolecular Engineering
Biosensor Technology
Nucleic Acid Diagnostics

Background:

Nucleic acid biomarkers are crucial for disease diagnosis and monitoring.
Developing efficient methods for immobilizing nucleic acid probes on sensor surfaces is essential for sensitive detection.

Purpose of the Study:

To design and implement bifunctional chimeric biomolecules for nucleic acid detection.
To create a versatile platform for immobilizing antisense oligonucleotide (ASO) probes onto solid substrates.

Main Methods:

Utilized a gold-binding peptide (AuBP1) for immobilizing probes on gold substrates.
Employed surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) for analysis.
Investigated the effect of steric hindrance on probe assembly and target capture.

Main Results:

Demonstrated sequential biomolecular assembly of solid-binding peptide-antisense oligonucleotide (SBP-ASO) constructs.
Observed that reduced probe packing density enhances target capture efficacy.
Noted viscoelastic changes in SBP-PNA layers upon DNA target capture, indicating increased flexibility.

Conclusions:

The bifunctional chimeric constructs offer a dual function for specific recognition and probe immobilization.
The modular design allows for facile functionalization of diverse solid substrates.
This approach provides a versatile platform for developing advanced nucleic acid detection systems.