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Related Experiment Video

Updated: Sep 23, 2025

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A Simple and Efficient Strategy for Preparing a Cell-Spheroid-Based Bioink.

Weiyan Sun1, Jiahui Zhang2, Yechi Qin2

  • 1Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, 200433, P. R. China.

Advanced Healthcare Materials
|May 11, 2022
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel hydrogel platform for creating cell-spheroid bioinks. This method efficiently produces adipose-derived stem cell spheroids for bioprinting, enhancing tissue engineering and cartilage regeneration.

Keywords:
bioinksbioprintingcell spheroidschondroidsorganoids

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Cell spheroids are crucial for mimicking physiological conditions in tissue engineering.
  • Current methods for preparing cell-spheroid bioinks face challenges in efficiency, printability, and spheroid fusion.
  • Adipose-derived stem cells (ASCs) are a valuable cell source for regenerative applications.

Purpose of the Study:

  • To develop an "all-in-one" platform for efficient cell-spheroid bioink preparation using a novel hydrogel.
  • To improve the printability and spheroid fusion ability of cell-spheroid-based bioinks.
  • To demonstrate the utility of the developed bioink for fabricating functional tissue-engineered constructs.

Main Methods:

  • Fabrication of a poly(N-isopropylacrylamide) (PNIPAAm)-based porous hydrogel with redox/thermo-responsiveness.
  • Utilizing the hydrogel's nonadhesive porous structure for high-yield ASC spheroid formation.
  • Employing the hydrogel as a carrier for spheroid transfer and as a temperature-sensitive bioprinting medium.

Main Results:

  • The hydrogel platform enabled efficient preparation of adipose-derived stem cell spheroids with high yield and good biological preservation.
  • The resulting cell-spheroid bioink exhibited favorable printability and enhanced spheroid fusion capability.
  • Bioprinted constructs demonstrated superior chondrogenic differentiation and feasibility for creating custom-shaped mature cartilage.

Conclusions:

  • The developed PNIPAAm-based hydrogel serves as an effective "all-in-one" platform for cell-spheroid bioink preparation.
  • This strategy offers a simple, efficient, and standardized approach for producing advanced tissue-engineered constructs.
  • The method facilitates accelerated maturation and functionalization of engineered tissues, particularly cartilage.