Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

2.8K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.8K
IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

12.6K
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
12.6K
Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

2.5K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.5K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

7.7K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.7K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.8K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.8K
Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

6.0K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
6.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advancing mechanobiology from single molecules to complex cellular systems.

Nature nanotechnology·2026
Same author

Monitoring Membrane Protein Folding Assisted by Insertases and Translocases Using AFM-Based Single-Molecule Force Spectroscopy.

Chemical reviews·2026
Same author

Evaluating Force Matching as a Parametrization Strategy for the CHARMM36m Force Field Using Phosphorylation.

The journal of physical chemistry. B·2026
Same author

Activation mechanism of the full-length histidine kinase LvrB from pathogenic Leptospira.

Nature communications·2026
Same author

Cell-type-targeted mitochondrial transplantation rescues cell degeneration.

Nature·2026
Same author

Cell viscosity influences haematogenous dissemination and metastatic extravasation of tumour cells.

Nature materials·2026
Same journal

Kat5 deficiency in alveolar type II cells licenses STAT6-driven glycolytic reprogramming and pulmonary fibrosis.

Nature communications·2026
Same journal

Continuous nonthermal slab gap formed by progressive tearing beneath Northeast Asia.

Nature communications·2026
Same journal

Zeolitic isolated protonic acid sites-mediated NH<sub>3</sub> storage for robust NO<sub>x</sub> removal.

Nature communications·2026
Same journal

Coaxially nested component with asymmetric fiber resonant cavity and separation membrane for gaseous and dissolved gases detection.

Nature communications·2026
Same journal

Near-unity charge readout signal in a nonlinear resonator without matching the sensor dissipation.

Nature communications·2026
Same journal

Prokaryotic Schlafen proteins cleave tRNAs during type III CRISPR immunity.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Sep 23, 2025

Density Gradient Multilayered Polymerization DGMP: A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering
12:54

Density Gradient Multilayered Polymerization DGMP: A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering

Published on: February 12, 2013

12.6K

Gasdermin-A3 pore formation propagates along variable pathways.

Stefania A Mari1, Kristyna Pluhackova2, Joka Pipercevic3

  • 1Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich, 4058, Basel, Switzerland.

Nature Communications
|May 11, 2022
PubMed
Summary
This summary is machine-generated.

Gasdermins form pores during pyroptosis, a cell death process. This study reveals gasdermin-A3 assembly on membranes and pore formation via adaptable structures, ensuring robust cellular responses.

More Related Videos

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients
05:57

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

Published on: May 24, 2019

6.7K
Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
11:31

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Published on: May 12, 2023

1.2K

Related Experiment Videos

Last Updated: Sep 23, 2025

Density Gradient Multilayered Polymerization DGMP: A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering
12:54

Density Gradient Multilayered Polymerization DGMP: A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering

Published on: February 12, 2013

12.6K
3D Analysis of Multi-cellular Responses to Chemoattractant Gradients
05:57

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

Published on: May 24, 2019

6.7K
Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
11:31

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Published on: May 12, 2023

1.2K

Area of Science:

  • Cellular Biology
  • Molecular Mechanisms
  • Immunology

Background:

  • Gasdermins are key effectors of pyroptosis, an inflammatory cell death pathway.
  • Gasdermin N-terminal domains oligomerize to form lytic pores in cell membranes.
  • The precise mechanisms of gasdermin assembly and pore formation remain largely unelucidated.

Purpose of the Study:

  • To elucidate the dynamic assembly and pore-forming mechanisms of gasdermins.
  • To characterize the structural basis of gasdermin-mediated membrane permeabilization.

Main Methods:

  • High-resolution time-lapse atomic force microscopy (AFM) of mouse gasdermin-A3.
  • Molecular dynamics (MD) simulations to investigate protein-membrane interactions.

Main Results:

  • Gasdermin-A3 oligomers assemble and remain mobile on the membrane surface.
  • Membrane-inserted gasdermin-A3 forms pores with variable oligomeric stoichiometries and shapes.
  • Amphiphilic β-hairpins and adaptable head domains stabilize pore conformations and facilitate membrane insertion.

Conclusions:

  • Gasdermin pore formation proceeds through multiple parallel pathways, not a single vertical collapse.
  • This multi-pathway mechanism ensures a robust and reliable cellular response during pyroptosis.
  • The findings provide novel insights into the dynamic nature of gasdermin pore assembly.