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Related Experiment Video

Updated: Sep 23, 2025

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

Extricating human tumour immune alterations from tissue inflammation.

Florian Mair¹, Jami R Erickson^1,2, Marie Frutoso¹

¹Fred Hutchinson Cancer Research Center, Vaccine and Infectious Disease Division, Seattle, WA, USA.

|May 11, 2022

Summary

This summary is machine-generated.

Researchers identified a unique regulatory T (Treg) cell population in head and neck cancers. These ICOS+IL1R1+ Treg cells are expanded and highly suppressive, offering new insights into tumor immunology and immunotherapy targets.

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Predictive Immune Modeling of Solid Tumors

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Published on: February 25, 2020

Area of Science:

Immunology
Oncology
Single-cell analysis

Background:

Immunotherapies show promise in cancer treatment but face challenges due to targeting pathways present in healthy tissues.
Understanding tumor-unique immune alterations is limited by knowledge gaps in inflamed tissue immune cell populations.

Purpose of the Study:

To identify tumor-enriched immune alterations by comparing immune infiltrates in head and neck squamous cell carcinomas with matched inflamed tissues.
To characterize novel immune cell populations and interactions within the tumor microenvironment.

Main Methods:

Utilized complementary single-cell analysis approaches to interrogate immune infiltrates.
Conducted computational analysis to identify tumor-enriched immune cell interactions.
Characterized regulatory T (Treg) cells based on co-expression of ICOS and IL-1 receptor type 1 (IL1R1).

Main Results:

Revealed significant overlap in immune cell composition and phenotype between tumor and inflamed tissues.
Identified a large population of ICOS+IL1R1+ Treg cells highly enriched in tumors.
Demonstrated that these intratumoral IL1R1+ Treg cells exhibit recent antigen response, clonal expansion, and superior suppressive function compared to IL1R1- Treg cells.

Conclusions:

Extensive immunological congruence exists between inflamed tissues and tumors, alongside tumor-specific changes.
A novel population of intratumoral IL1R1+ Treg cells with potent suppressive function was identified.
This study provides a framework for distinguishing disease-specific immune changes from general inflammation-associated patterns, aiding immunotherapy development.