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Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Published on: October 1, 2016
A "Turn-On" fluorescent probe for sensitive and selective detection of fluoride ions based on aggregation-induced
Man Du1, Baolong Huo1, Mengwen Li1
1Department of Chemistry and Chemical Engineering, School of Chemistry and Biological Engineering, University of Science and Technology Beijing Beijing 100083 China yxyang@ustb.edu.cn liujm@ustb.edu.cn.
Abstract:
Based on the fluorophore of 2-(2'-hydroxyphenyl)benzothiazole (HBT) with aggregation-induced emission (AIE) properties, a highly selective and sensitive fluorescent probe PBT towards F- was investigated. "Turn-On" fluorescence type signaling was realized by employing fluoride-selective cleavage of the latent thiophosphinated probe in mixed aqueous media. The probe is designed in such a way that the excited state intramolecular proton transfer (ESIPT) of the HBT moiety becomes blocked. The chemodosimetric approach of F- to the probe results in the recovery of the ESIPT by removal of a free AIE-active HBT moiety through a subsequent hydrolysis process. The F- detection limit of the probe was 3.8 nM in the dynamic range of 0.5 μM to 10 μM. In addition, the proposed probe has been used to detect F- in water samples and toothpaste samples with satisfying results.
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