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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
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Activatable NIR-II organic fluorescent probes for bioimaging.

Xiaoning Zhang1, Shasha Li1, Huizhen Ma2

  • 1Tianjin Key Laboratory of Brain Science and Neural Engineering, Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin 300072, China.

Theranostics
|May 13, 2022
PubMed
Summary
This summary is machine-generated.

Activatable near-infrared II (NIR-II) fluorescence probes enable noninvasive deep tissue imaging. These organic probes offer tunable properties and biocompatibility for detecting various pathological changes, showing promise for clinical use.

Keywords:
NIR-II fluorescencebioimagingorganic fluorescent probespathological changesresponsive probes

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Area of Science:

  • Biomedical Optics
  • Molecular Imaging
  • Nanotechnology

Background:

  • Near-infrared II (NIR-II) imaging offers deep tissue visualization with high resolution.
  • Activatable fluorescence probes are crucial for reporting pathological changes.
  • Organic fluorescent probes show significant advancements in NIR-II imaging.

Purpose of the Study:

  • To review the design, synthesis, and applications of activatable NIR-II fluorescence probes.
  • To highlight the potential of these probes for noninvasive pathological inspection.
  • To discuss the prospects of NIR-II imaging for clinical translation.

Main Methods:

  • Review of recent literature on activatable NIR-II fluorescence probes.
  • Analysis of probe design strategies and synthetic approaches.
  • Evaluation of probe performance in imaging various biomarkers.

Main Results:

  • Activatable NIR-II probes exhibit tunable physicochemical and optical properties.
  • These probes demonstrate excellent biocompatibility and processability.
  • Applications include imaging reactive oxygen species (ROS), pH, hypoxia, and enzymes.

Conclusions:

  • Activatable NIR-II fluorescence probes are powerful tools for noninvasive deep tissue imaging.
  • Their versatility in detecting diverse pathological markers is highlighted.
  • The technology shows significant promise for future clinical applications.