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Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Published on: July 19, 2016

Enhanced distance-dependent fluorescence quenching using size tuneable core shell silica nanoparticles.

Mohamed M Elsutohy^1,2, Amjad Selo¹, Veeren M Chauhan¹

¹Boots Science Building, School of Pharmacy, University Park Campus, University of Nottingham Nottingham NG7 2RD UK jon.aylott@nottingham.ac.uk.

|May 13, 2022

Summary

We developed a protocol for synthesizing size-tunable silica nanoparticles (SNPs) for biological sensors. These fluorescent SNPs enable ratiometric pH sensing and demonstrate enhanced distance-dependent fluorescence quenching for novel sensor applications.

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Area of Science:

Nanotechnology
Materials Science
Biomedical Engineering

Background:

Silica nanoparticles (SNPs) are versatile platforms for sensing, drug delivery, and imaging due to tunable properties.
Developing reproducible protocols for size-controlled SNP synthesis is crucial for advanced applications.

Purpose of the Study:

To establish a protocol for synthesizing size-tuneable silica nanoparticles (SNPs) ranging from 20 nm to 500 nm.
To create fluorescent SNPs for fabricating ratiometric, pH-sensitive nanosensors.
To investigate distance-dependent fluorescence quenching using core-shell SNPs.

Main Methods:

Optimized experimental components for synthesizing size-tuneable SNPs.
Covalently linked fluorophores (Oregon Green, FAM, TAMRA) to SNPs using APTES for fluorescent sensors.
Synthesized core-shell SNPs with TAMRA cores and varied silica shell thicknesses to study quenching with BHQ2®.

Main Results:

Successfully synthesized size-tuneable SNPs (20-500 nm) and fluorescent SNPs.
Fabricated ratiometric, fluorescent, pH-sensitive nanosensors (75 nm) with a dynamic range of pH 3.5-7.5.
Observed significant fluorescence quenching over greater distances than FRET, indicating potential for novel sensor designs.

Conclusions:

The developed protocol enables reproducible production of size-tunable SNPs.
These SNPs are suitable for creating advanced fluorescent nanosensors for biological applications.
The findings advance the understanding of distance-dependent fluorescence quenching for sensor development.