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Targeting HMGA protein inhibits retinoblastoma cell proliferation
Akilandeswari Balachandran1, Ajit Zambre2, Jagjot Singh Kainth2,3
1Department of Nanobiotechnology, Vision Research Foundation, Kamalnayan Bajaj Institute for Research in Vision and Ophthalmology Chennai India drkk@snmail.org drkrishnakumar_2000@yahoo.com.
RSC Advances
|May 13, 2022
Summary
We developed a new method to link HMGA2 siRNA and aptamers to nucleolin aptamers and antibodies. These novel conjugates effectively reduce retinoblastoma cell growth.
Area of Science:
- Biochemistry
- Molecular Biology
- Oncology
Background:
- Retinoblastoma is a pediatric eye cancer with limited treatment options.
- High-mobility group AT-hook 2 (HMGA2) is implicated in cancer progression.
- Targeted therapies are needed to improve retinoblastoma treatment outcomes.
Purpose of the Study:
- To develop a novel synthetic strategy for creating targeted drug conjugates.
- To evaluate the efficacy of HMGA2 siRNA and aptamer conjugates in inhibiting retinoblastoma cell proliferation.
Main Methods:
- Synthesized conjugates linking HMGA2 siRNA and HMGA aptamer to nucleolin aptamer and antibody.
- Utilized a novel conjugation strategy for targeted delivery.
- Assessed the impact of conjugates on retinoblastoma cell proliferation in vitro.
Main Results:
- Successfully synthesized novel HMGA2 siRNA and aptamer conjugates.
- Demonstrated significant inhibition of retinoblastoma cell proliferation by the conjugates.
- Validated the targeted delivery approach using nucleolin aptamer and antibody.
Conclusions:
- The novel synthetic strategy provides a promising platform for developing targeted cancer therapies.
- HMGA2-targeted conjugates show potential for treating retinoblastoma.
- Further research is warranted to explore the clinical applications of these conjugates.
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