Related Experiment Video

Updated: Sep 23, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.7K

LncRNA HAND2-AS1 attenuates glioma cell proliferation, invasion and migration by targeting CDK6

Songlin Liu1, Yifeng Li2

  • 1Department of Radiology, Xiangya Hospital, Central South University, Changsha, China.

Neurological Research
|May 13, 2022
PubMed
Abstract

Insights

Long non-coding RNA HAND2-AS1 is downregulated in glioma. Its overexpression inhibits glioma cell proliferation, invasion, and migration, suggesting HAND2-AS1 acts as an anti-tumorigenic factor by regulating CDK6.

Area of Science:

  • Molecular biology
  • Oncology
  • Genetics

Background:

  • Long non-coding RNA (lncRNA) HAND2-AS1 has known roles in various cancers.
  • The specific function of HAND2-AS1 in glioma remains largely unexplored.

Purpose of the Study:

  • To investigate the role of HAND2-AS1 in glioma cell proliferation, invasion, and migration.
  • To elucidate the molecular mechanism by which HAND2-AS1 influences glioma progression.

Main Methods:

  • Quantitative polymerase chain reaction (qPCR) to assess HAND2-AS1 and CDK6 expression.
  • Western blot analysis for CDK6 protein levels.
  • Cell Counting Kit-8 (CCK-8) and Transwell assays to evaluate cell proliferation, invasion, and migration.

Main Results:

  • HAND2-AS1 expression was significantly reduced in glioma tissues and cell lines.
  • Overexpression of HAND2-AS1 suppressed glioma cell proliferation, invasion, and migration.
  • Increased CDK6 expression partially reversed the inhibitory effects of HAND2-AS1.

Conclusions:

  • LncRNA HAND2-AS1 exhibits anti-tumorigenic properties in glioma.
  • HAND2-AS1 negatively regulates CDK6 expression, thereby inhibiting glioma progression.
Keywords:
CDK6GliomainvasionlncRNA HAND2-AS1migrationproliferation

More Related Videos

Real-Time Quantitative Measurement of Tumor Cell Migration and Invasion Following Synthetic mRNA Transfection11:00

Real-Time Quantitative Measurement of Tumor Cell Migration and Invasion Following Synthetic mRNA Transfection

Published on: June 23, 2023

1.3K
Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy09:40

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Published on: October 4, 2019

5.7K

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.0K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.2K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.0K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.2K
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.5K