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ADAPTOR PROTEIN Ruk/CIN85REGULATES REDOX BALANCE IN 4T1MOUSE BREAST CANCER CELLS EXPOSED TO PLASMIN(OGEN).

I R Horak1, N V Latyshko1, O O Hudkova1

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Experimental Oncology
|May 13, 2022
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Adaptor protein Ruk/CIN85 enhances breast cancer cell survival against plasmin-induced oxidative stress by boosting antioxidant defenses. This suggests Ruk/CIN85 as a potential therapeutic target to overcome cancer cell resistance to ROS-mediated apoptosis.

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Area of Science:

  • Oncology
  • Cell Biology
  • Biochemistry

Background:

  • Cell surface plasmin plays a role in tumor growth, metastasis, and invasion.
  • Plasmin induces cell death via reactive oxygen species (ROS) generation, but cancer cells develop resistance mechanisms.
  • Understanding these adaptive mechanisms is crucial for developing effective cancer therapies.

Purpose of the Study:

  • To investigate the role of adaptor protein Ruk/CIN85 in regulating viability and redox balance in breast adenocarcinoma cells exposed to plasmin(ogen).
  • To determine if Ruk/CIN85 influences cancer cell resistance to plasmin-induced cytotoxicity and ROS generation.

Main Methods:

  • Overexpression of Ruk/CIN85 in mouse 4T1 breast cancer cells (RukUp subline) and control (Mock subline).
  • Treatment with Glu-plasminogen and monitoring of plasminogen to plasmin conversion.
  • Assessment of cell survival (MTT-test), proliferation (colony formation assay), ROS generation (flow cytometry), and antioxidant enzyme activities (catalase, glutathione peroxidase, superoxide dismutase).

Main Results:

  • Plasminogen conversion to plasmin on cancer cell surfaces was confirmed in a uPA-dependent manner.
  • Plasmin(ogen) reduced proliferation and survival in both cell lines, but RukUp cells showed higher resistance.
  • RukUp cells exhibited reduced ROS generation and H2O2 accumulation, alongside increased glutathione peroxidase activity, indicating enhanced antioxidant defense.

Conclusions:

  • Adaptor protein Ruk/CIN85 is involved in maintaining redox homeostasis in cancer cells exposed to plasmin(ogen).
  • Ruk/CIN85 promotes cancer cell adaptation to ROS, enhancing survival by modulating antioxidant systems.
  • Targeting Ruk/CIN85 could potentially reduce cancer cell resistance to ROS-mediated apoptosis.