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Circular RNA_0120376 regulates microRNA-148b-3 and centrosomal protein 55 to promote non-small cell lung cancer

Tiantian Du1, Shenni Yi1, Yuanyuan Wang1

  • 1Department of Respiratory and Critical Medicine, Yantai Yuhuangding Hospital, Yantai, China.

Bioengineered
|May 13, 2022
PubMed

Insights

Circular RNAs (circRNAs) promote non-small cell lung cancer (NSCLC) malignancy by upregulating CEP55 via sponging miR-148b-3p. Circ_0120376 may serve as a diagnostic biomarker and therapeutic target for NSCLC patients.

Area of Science:

  • Molecular Biology
  • Oncology
  • RNA Biology

Background:

  • Circular RNAs (circRNAs) are key regulators in biological processes.
  • Dysregulation of circRNAs is implicated in various cancers, including NSCLC.
  • The specific role of circ_0120376 in NSCLC remains largely unexplored.

Purpose of the Study:

  • To investigate the role and mechanism of circ_0120376 in non-small cell lung cancer (NSCLC).
  • To explore the potential of circ_0120376 as a diagnostic biomarker and therapeutic target for NSCLC.

Main Methods:

  • qRT-PCR to quantify circ_0120376, miR-148b-3p, and CEP55 mRNA expression.
  • Cell proliferation, apoptosis, migration, and invasion assays (CCK-8, colony formation, flow cytometry, Transwell).
  • Dual-luciferase reporter and RIP assays to confirm molecular interactions.
  • Western blot to assess protein expression levels.

Main Results:

  • Circ_0120376 was significantly overexpressed in NSCLC tissues and correlated with advanced T stage and lymph node metastasis.
  • Overexpression of circ_0120376 promoted NSCLC cell proliferation, migration, and invasion while inhibiting apoptosis.
  • Circ_0120376 acted as a molecular sponge for miR-148b-3p, thereby increasing CEP55 expression.
  • Knockdown of circ_0120376 suppressed NSCLC cell malignancy.

Conclusions:

  • Circ_0120376 promotes NSCLC progression through the ceRNA mechanism by regulating the miR-148b-3p/CEP55 axis.
  • Circ_0120376 presents a potential diagnostic biomarker and therapeutic target for NSCLC.
Keywords:
CEP55Circ_0120376NSCLCmiR-148b-3p

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