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Updated: Sep 23, 2025
Rescue of Recombinant Newcastle Disease Virus from cDNA
Published on: October 11, 2013
A rescued NADC30-like virus by reverse genetic manipulation exhibits moderate virulence and a promising application
Zhengda Cao1, Jinxia Chen2, Liwei Li2
1Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China; Shandong Agricultural University, Shandong, 271018, China.
Abstract:
NADC30-like porcine reproductive and respiratory syndrome virus (PRRSV), which is highly homologous to the NADC30 strain isolated in the United States. The NADC30-like PRRSV was first reported in 2014 in China, where it spread and gradually caused an epidemic. Currently, growing research has shown that NADC30-like strains have greater propensity to recombine with other PRRSV strains, particularly the PPRSV vaccine virus used clinically, making the prevention and control of PRRSV highly complex. To carry out an in-depth molecular biology and virulence analysis, a full-length infectious clone of the NADC30-like strain was successfully constructed and rescued by reverse genetic manipulation. The rescued virus, rZJqz, was indistinguishable from its parental virus, ZJqz21, based on virological characteristics. Further animal experiments demonstrated that rZJqz retained similar pathogenicity and induced the typical clinical symptoms and viral shedding observed in the ZJqz21 challenge model. Together, these results provide a useful tool for further study of the biological characteristics and pathogenicity of NADC30-like strains. Moreover, these findings also provide a solid foundation for studying the recombination of different PRRSVs and developing new and effective universal vaccines in the future.
Insights
Researchers created a viable clone of the NADC30-like Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). This tool aids in studying PRRSV characteristics and developing new vaccines.
Area of Science:
- Veterinary Virology
- Molecular Biology
- Animal Health
Background:
- NADC30-like Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) strains are prevalent in China, showing high homology to US-isolated strains.
- These strains exhibit a significant propensity for recombination with other PRRSV strains, including vaccine viruses, complicating disease control.
- The increasing complexity of PRRSV prevention and control necessitates deeper molecular and virulence analyses.
Purpose of the Study:
- To construct a full-length infectious clone of the NADC30-like PRRSV strain.
- To analyze the molecular biology and pathogenicity of the NADC30-like PRRSV.
- To provide a tool for future research on PRRSV biological characteristics and vaccine development.
Main Methods:
- Construction and rescue of a full-length infectious clone of NADC30-like PRRSV using reverse genetics.
- Virological characterization of the rescued virus (rZJqz) compared to its parental strain (ZJqz21).
- Animal experiments to assess pathogenicity, clinical symptoms, and viral shedding of the rescued virus.
Main Results:
- A rescued virus, rZJqz, was successfully generated and found indistinguishable from the parental ZJqz21 strain in virological characteristics.
- The rescued virus rZJqz demonstrated similar pathogenicity to ZJqz21, inducing typical clinical symptoms and viral shedding.
- The study provides a viable tool for investigating NADC30-like PRRSV strains.
Conclusions:
- The rescued NADC30-like PRRSV clone (rZJqz) serves as a valuable tool for studying viral characteristics and pathogenicity.
- These findings lay the groundwork for understanding PRRSV recombination and developing novel, effective universal vaccines.
- Further research using this infectious clone can advance strategies for PRRSV prevention and control.
