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Structure-function conservation between the methyltransferases SETD3 and SETD6.

Lee Admoni-Elisha1, Elina Abaev-Schneiderman1, Ofir Cohn1

  • 1The Shraga Segal Department of Microbiology, Immunology and Genetics, Israel; The National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, P.O.B. 653, Be'er-Sheva, 84105, Israel.

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Summary
This summary is machine-generated.

SETD6 and SETD3 protein methyltransferases share functions and interact with transcription proteins. Double knockout cells show increased apoptosis, suggesting these proteins inhibit cell death.

Keywords:
MethyltransferasesSETD3SETD6

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • SETD6 and SETD3 are protein lysine methyltransferases with high structural similarity.
  • Their structural similarity suggests potential shared cellular functions.
  • Investigating the functional relationship between SETD6 and SETD3 is crucial for understanding their roles.

Purpose of the Study:

  • To explore the functional relationship between SETD6 and SETD3.
  • To identify shared interacting proteins and their functions.
  • To investigate the role of SETD6 and SETD3 in apoptosis regulation.

Main Methods:

  • Proteomic screening to identify shared interacting proteins.
  • Gene Ontology (GO) analysis to determine enriched functions of interacting proteins.
  • RNA sequencing (RNA-seq) to analyze gene expression changes in SETD6 and SETD3 knockout cells.
  • Assessment of apoptosis levels in knockout cells.

Main Results:

  • Identified 52 shared interacting proteins between SETD6 and SETD3.
  • GO analysis revealed enrichment of transcription-related proteins among shared interactors.
  • RNA-seq data showed approximately 100 up-regulated and down-regulated shared genes in single knockouts.
  • Double knockout cells exhibited significant changes in a distinct set of genes enriched for apoptosis pathways.
  • Double knockout cells displayed significantly elevated levels of apoptosis.

Conclusions:

  • SETD6 and SETD3 share interacting proteins involved in transcription.
  • SETD6 and SETD3 function together to inhibit apoptosis.
  • There is a strong functional link between SETD6 and SETD3 in the regulation of apoptosis.