Related Experiment Video
Updated: Sep 23, 2025

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
Bio-augmentation with dissimilatory nitrate reduction to ammonium (DNRA) driven sulfide-oxidizing bacteria enhances
Panqing Qi1, Dejun Sun2, Gaixin Zhang1
1Shandong Key Laboratory of Environmental Processes and Health, School of Environmental Science and Engineering, Shandong University, Qingdao 266237, PR China.
Abstract:
Biological souring (producing sulfide) is a global challenge facing anaerobic water bodies, especially the oil reservoir fluids. Nitrate injection has demonstrated great potential in souring control, and dissimilatory nitrate reduction to ammonium (DNRA) bacteria was proposed to play crucial roles in the process. How to durably control souring with nitrate amendment, however, remains undiscovered. Herein, Gordonia sp. TD-4, a DNRA-driven sulfide-oxidizing bacterium, was used to elucidate the effects of bio-augmentation with DNRA bacteria on the durability of nitrate-mediated souring control. The results revealed that nitrate amendment combined with bio-augmentation with TD-4 after souring could effectively control souring and enhance the durability of nitrate-mediated souring control, while nitrate amendment before souring failed to persistently control souring. Nitrate amendment before and after souring resulted in different evolution dynamics of nitrate-reducing bacteria. Denitrifying bacteria were enriched in reactors amended with nitrate before souring or in dissolved sulfide exhausted reactors amended with nitrate after souring. The heterotrophic denitrifying activity of denitrifying bacteria, however, decreased the durability of nitrate-mediated souring control. Comparative and functional genomics analysis identified potential niche adaptation mechanisms (autotrophic and heterotrophic nitrate/nitrite reduction, including DNRA and denitrification) of predominant SRB in nitrate-amended environments, which were responsible for the rapid resumption of sulfide accumulation after the depletion of nitrate and nitrite. Pulsed injection of nitrate combined with bio-augmentation with DNRA-driven sulfide-oxidizing bacteria was proposed as a potential method to enhance the durability of nitrate-mediated souring control. The findings were innovatively applied to simultaneous bio-demulsification and souring control of emulsified and sour produced water from the petroleum industry.
More Related Videos
08:05Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
Published on: October 7, 2020
08:13A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Related Concept Videos
Inorganic Nitrogen Assimilation
Metabolism of Chemolithotrophs
Bioremediation
Sulfur Assimilation
Environmental Applications of Microorganisms
2° Amines to N-Nitrosamines: Reaction with NaNO2