Related Experiment Video
Updated: Sep 23, 2025
Unbiased Deep Sequencing of RNA Viruses from Clinical Samples
Published on: July 2, 2016
ReadItAndKeep: rapid decontamination of SARS-CoV-2 sequencing reads
Martin Hunt1,2, Jeremy Swann2, Bede Constantinides2
1EMBL-EBI, Cambridge CB10 1SD, UK.
Summary:
Viral sequence data from clinical samples frequently contain contaminating human reads, which must be removed prior to sharing for legal and ethical reasons. To enable host read removal for SARS-CoV-2 sequencing data on low-specification laptops, we developed ReadItAndKeep, a fast lightweight tool for Illumina and nanopore data that only keeps reads matching the SARS-CoV-2 genome. Peak RAM usage is typically below 10 MB, and runtime less than 1 min. We show that by excluding the polyA tail from the viral reference, ReadItAndKeep prevents bleed-through of human reads, whereas mapping to the human genome lets some reads escape. We believe our test approach (including all possible reads from the human genome, human samples from each of the 26 populations in the 1000 genomes data and a diverse set of SARS-CoV-2 genomes) will also be useful for others.
Availability And Implementation:
ReadItAndKeep is implemented in C++, released under the MIT license, and available from https://github.com/GenomePathogenAnalysisService/read-it-and-keep.
Supplementary Information:
Supplementary data are available at Bioinformatics online.
