Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Sep 23, 2025

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
10:19

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Published on: April 8, 2017

17.6K

Identifying the Caenorhabditis elegans vulval transcriptome.

Qi Zhang1, Heather Hrach2,3, Marco Mangone2,3

  • 1Department of Translational Medical Science, Institute of Biosciences and Technology, Texas A&M Health Science Center, Texas A&M University, Houston, TX 77030, USA.

G3 (Bethesda, Md.)
|May 13, 2022
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aging, Dauer, and Stature Phenotypes Are Conferred by Structure-Directed Missense Mutations in the Endogenous AGE-1/Phosphatidylinositol 3-Kinase Catalytic Subunit.

Aging cell·2026
Same author

Canonical and noncanonical Hippo signaling in C. elegans.

Genetics·2026
Same author

Aging, dauer, and stature phenotypes are conferred by structure-directed missense mutations in the endogenous AGE-1/phosphatidylinositol 3-kinase catalytic subunit.

bioRxiv : the preprint server for biology·2026
Same author

The Caenorhabditis Genetics Center Curated Special Collections: a guide to protein degradation systems.

Genetics·2026
Same author

An AI-guided framework reveals conserved features governing microRNA strand selection.

Nucleic acids research·2026
Same author

The Ral small GTPase is an essential regulator of Exocyst complex function in secretion.

bioRxiv : the preprint server for biology·2025

Researchers identified the vulva-specific transcriptome in Caenorhabditis elegans, revealing key genes for organogenesis. This discovery provides a foundation for understanding vulval development and related functions.

Area of Science:

  • Developmental Biology
  • Genomics
  • Organogenesis

Background:

  • Vulval development in Caenorhabditis elegans is a well-established model for organogenesis.
  • This process involves precise cell fate patterning, lineage execution, and morphogenesis.
  • Current knowledge of the transcriptomic landscape during vulval development is limited.

Purpose of the Study:

  • To characterize the vulva-specific transcriptome in Caenorhabditis elegans.
  • To identify genes involved in vulval development and organogenesis.
  • To provide a resource for future functional studies.

Main Methods:

  • Tissue-specific mRNA harvesting using immunoprecipitation of epitope-tagged poly(A) binding protein (PAB-1).
  • Heterologous expression of PAB-1 using a vulva-specific promoter driving GFP.
Keywords:
Caenorhabditis elegansF27A3.4lag-1lin-31mbl-1shc-1toe-1vulval transcriptome

More Related Videos

Hand Dissection of Caenorhabditis elegans Intestines
05:41

Hand Dissection of Caenorhabditis elegans Intestines

Published on: September 13, 2022

3.6K
Purification and Analysis of Caenorhabditis elegans Extracellular Vesicles
09:30

Purification and Analysis of Caenorhabditis elegans Extracellular Vesicles

Published on: March 31, 2020

7.7K

Related Experiment Videos

Last Updated: Sep 23, 2025

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
10:19

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Published on: April 8, 2017

17.6K
Hand Dissection of Caenorhabditis elegans Intestines
05:41

Hand Dissection of Caenorhabditis elegans Intestines

Published on: September 13, 2022

3.6K
Purification and Analysis of Caenorhabditis elegans Extracellular Vesicles
09:30

Purification and Analysis of Caenorhabditis elegans Extracellular Vesicles

Published on: March 31, 2020

7.7K
  • Transcriptome analysis and validation using promoter-GFP reporters and endogenous protein tagging.

    • Main Results:

    • A small but tightly interconnected vulva-specific transcriptome was identified.
    • Several genes with known roles in vulval development were found within this set.
    • Validation confirmed expression patterns for identified genes, including dynamic expression of lag-1.

    Conclusions:

    • The study successfully identified a vulva-specific transcriptome in C. elegans.
    • This resource serves as a foundation for exploring gene functions in vulval organogenesis.
    • Further research can leverage this transcriptome to uncover novel mechanisms of development.