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Neutralization Assay for SARS-CoV-2 Infection: Plaque Reduction Neutralization Test
Sandra Westhaus1, Holger F Rabenau2
1Institue of Medical Viroogy, University of Frankfurt, Goethe-University, Frankfurt, Germany. Sandra.Westhaus@uk-essen.de.
Methods in Molecular Biology (Clifton, N.J.)
|May 13, 2022
Summary
This study details the plaque reduction neutralization test (PRNT) for detecting SARS-CoV-2 antibodies in patient samples. This method helps assess immune response after infection or vaccination.
Area of Science:
- Immunology
- Virology
- Diagnostic Assays
Background:
- Neutralization assays are crucial for research and diagnostics.
- They detect neutralizing antibodies and assess protective antibody titers post-infection or vaccination.
Purpose of the Study:
- To describe a conventional plaque reduction neutralization test (PRNT).
- To evaluate the presence of SARS-CoV-2 antibodies in patient serum or plasma samples.
Main Methods:
- Conventional plaque reduction neutralization test (PRNT).
- Analysis of patient serum or plasma samples.
Main Results:
- The PRNT method was successfully applied.
- Detection of antibodies against SARS-CoV-2 was achieved.
Conclusions:
- The plaque reduction neutralization test is a viable method for detecting SARS-CoV-2 antibodies.
- This assay aids in understanding immune responses to SARS-CoV-2.
Keywords:
Caco-2 cell lineNeutralizing antibodyNeutralizing assayPlaque reduction neutralization testSARS-CoV-2
