Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Unbiased stereologic estimation of surface density in bone using vertical sections.

A Vesterby, J Kragstrup, H J Gundersen

    Bone
    |January 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Nurse navigation is helpful for cancer patients, but with some restrictions.

    EJC supplements : EJC : official journal of EORTC, European Organization for Research and Treatment of Cancer ... [et al.]·2015
    Same author

    Mast cell densities in bronchial biopsies and small airways are related.

    Journal of clinical pathology·2011
    Same author

    Exercise on prescription: a randomized study on the effect of counseling vs counseling and supervised exercise.

    Scandinavian journal of medicine & science in sports·2008
    Same author

    Double lamellae in trabecular osteons: towards a new method for age estimation by bone microscopy.

    Homo : internationale Zeitschrift fur die vergleichende Forschung am Menschen·2007
    Same author

    Plasma 25-hydroxyvitamin D and not 1,25-dihydroxyvitamin D is associated with parathyroid adenoma secretion in primary hyperparathyroidism: a cross-sectional study.

    European journal of endocrinology·2006
    Same author

    Vitamin D status, seasonal variations, parathyroid adenoma weight and bone mineral density in primary hyperparathyroidism.

    Clinical endocrinology·2005

    This study introduces a new stereologic method using vertical sections for unbiased bone surface density estimation in iliac crest biopsies. This technique corrects for bone anisotropy, providing more accurate results for trabecular bone analysis.

    Area of Science:

    • Stereology
    • Bone Histomorphometry
    • Anisotropic Materials Analysis

    Background:

    • Conventional stereology for bone surface density in iliac crest biopsies yields biased results due to bone substructure anisotropy.
    • Biopsy sectioning parallel to the cylindrical axis contributes to these estimation biases.

    Purpose of the Study:

    • To describe the methodology and practical sampling procedure for vertical sections in stereologic analysis.
    • To obtain unbiased estimates of bone surface densities in iliac crest biopsies.
    • To evaluate the reliability and variance associated with the vertical sectioning method.

    Main Methods:

    • Utilized random anisotropic sections with a fixed "vertical" axis for unbiased surface area estimation.
    • Employed an anisotropic cycloid test system designed for vertical sections to estimate trabecular bone surface density.

    Related Experiment Videos

  • Analyzed 16 bone samples from eight deceased individuals (mean age 64 years).

    • Main Results:

    • The average surface density of trabecular bone was estimated at 2.8 +/- 0.4 mm⁻¹ (SD).
    • No systematic differences were found between paired bone specimens from the same individuals.
    • The majority of the observed variance in estimations was attributed to inter-individual differences.

    Conclusions:

    • Vertical sectioning provides an unbiased method for estimating surface densities, particularly for anisotropic structures like trabecular bone.
    • The cycloid test system is efficient and easy to use, ensuring unbiased surface density estimates.
    • Key requirements for accurate estimation include parallel sectioning to the vertical axis, random rotation, random biopsy positioning, and clear identification of the vertical axis.