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A method for preparing leucocyte depleted erythrocytes from rat blood
Journal of Immunological Methods
|January 1, 1978
Summary
This study details a Ficoll-Hypaque density gradient centrifugation method for purifying rat blood. The technique effectively removes over 99.8% of leukocytes while preserving erythrocytes and most leukocytes.
Area of Science:
- Hematology
- Cell Biology
- Biotechnology
Background:
- Leukocyte contamination can affect downstream cellular analysis.
- Efficient separation of blood components is crucial for research.
Purpose of the Study:
- To develop a reliable method for high-purity leukocyte depletion from rat blood.
- To optimize Ficoll-Hypaque density gradient centrifugation for blood cell separation.
Main Methods:
- Heparinized rat blood was centrifuged multiple times (2-3) using double discontinuous Ficoll-Hypaque density gradients.
- Density gradient centrifugation was employed to separate blood components based on their buoyant density.
Main Results:
- Achieved over 99.8% depletion of leukocytes from the blood samples.
- Successfully retrieved nearly all original erythrocytes and a significant portion of leukocytes.
Conclusions:
- The described Ficoll-Hypaque method provides highly effective leukocyte depletion from rat blood.
- This technique is valuable for obtaining purer erythrocyte populations and for research requiring minimal leukocyte contamination.