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Related Experiment Videos

Mast cell secretion: membrane events.

D Lagunoff, E Y Chi

    The Journal of Investigative Dermatology
    |July 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Stimulating secretion with A23187 reveals key events in granule exocytosis. Membrane changes, requiring ATP and protein sulfhydryl groups, precede fusion, independent of direct calcium effects on phospholipids.

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    Area of Science:

    • Cell Biology
    • Membrane Biology
    • Biochemistry

    Background:

    • Secretion involves granule exocytosis, a complex process regulated by intracellular calcium (Ca++).
    • The standard secretory pathway is often difficult to study in its terminal stages.
    • A23187 ionophore bypasses normal signaling, allowing direct investigation of the final exocytosis steps.

    Purpose of the Study:

    • To investigate the terminal events of the secretory process, specifically granule externalization via membrane fusion.
    • To identify the early membrane alterations preceding fusion during A23187-induced secretion.
    • To determine the cellular requirements for these pre-fusion membrane changes.

    Main Methods:

    • Utilized the ionophore A23187 to stimulate secretion, bypassing conventional Ca++ signaling pathways.

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  • Observed plasma membrane alterations, including bulges and intramembranous particle aggregation, using electron microscopy.
  • Assessed the dependence of these membrane changes on cellular ATP levels and intracellular protein sulfhydryl groups.

    • Main Results:

    • A23187-induced secretion allowed focused study of terminal exocytosis and membrane fusion.
    • Two distinct plasma membrane alterations—bulge formation and particle aggregation—were identified preceding fusion.
    • These membrane changes were dependent on cellular ATP and sensitive to reagents targeting protein sulfhydryl groups.
    • Direct effects of Ca++ on membrane phospholipids were not implicated in these alterations.

    Conclusions:

    • The study elucidates critical pre-fusion membrane events in exocytosis.
    • Cellular ATP and protein sulfhydryl groups are essential for initiating membrane changes leading to secretion.
    • The precise Ca++-sensitive components driving these membrane alterations remain unidentified, excluding microtubules and actin filaments.