Related Experiment Video

Updated: Sep 23, 2025

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

4.2K

Nucleic Acid Sensing Pathways in DNA Repair Targeted Cancer Therapy

Bingteng Xie1,2, Aiqin Luo1,2

  • 1School of Life Science, Beijing Institute of Technology, Beijing, China.

Frontiers in Cell and Developmental Biology
|May 13, 2022
PubMed

Insights

DNA damage repair in cancer cells affects treatment efficacy. Emerging research shows DNA damage triggers immune responses via nucleic acid sensing, influencing therapy outcomes and offering new cancer treatment strategies.

Area of Science:

  • Molecular Biology
  • Immunology
  • Oncology

Background:

  • DNA damage repair is crucial for genome stability and cancer treatment efficacy.
  • Cancer cells' ability to repair DNA damage impacts therapeutic outcomes.
  • Recent findings link DNA damage to innate and adaptive immune responses via nucleic acid sensing.

Purpose of the Study:

  • To review the molecular mechanisms of innate immune responses to DNA damage through nucleic acid-sensing pathways.
  • To summarize the impact of nucleic acid-sensing pathways on the DNA damage response (DDR).
  • To highlight the interplay between DDR and immune responses for improved cancer therapies.

Main Methods:

  • Literature review focusing on DNA damage, nucleic acid sensing, and immune response pathways.
  • Analysis of DNA sensing mechanisms (cGAS, TLR9, AIM2, DNA-PK, MRN).
  • Examination of RNA sensing mechanisms (TLR3/7/8, RIG-I-like receptors).

Main Results:

  • DNA damage activates innate immunity through various DNA and RNA sensing pathways.
  • These pathways modulate the DNA damage response (DDR).
  • The interplay between DDR and immune signaling is critical for therapeutic efficacy.

Conclusions:

  • Understanding the DDR-immune response crosstalk is essential for developing novel cancer therapies.
  • Targeting nucleic acid-sensing pathways can enhance antitumor immunity.
  • Harnessing immunomodulatory effects holds promise for improving cancer treatment strategies.
Keywords:
DDR inhibitorsDNA damage and repairimmunotherapyinnate immunitynucleic acid-sensing pathways

More Related Videos

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

3.7K
Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

10.1K

Related Concept Videos

Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
3.9K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
7.9K
Base Excision Repair01:54

Base Excision Repair

One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
23.1K
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
31.7K
DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.4K
Base-pairing and DNA Repair02:27

Base-pairing and DNA Repair

65.2K