Synthesis, characterization and osteogenesis of phosphorylated methacrylamide chitosan hydrogels
Huishang Yang1, Shenggui Chen1, Lei Liu1
1National Engineering Research Centre for Tissue Restoration and Reconstruction, School of Material Science and Engineering, South China University of Technology Guangzhou 510640 P. R. China shxt@scut.edu.cn.
RSC Advances
|May 13, 2022
Summary
Phosphorylated methacrylamide chitosan hydrogels show promise for bone tissue engineering. These scaffolds enhance osteoblast activity and mineralization, mimicking natural bone formation for potential bone repair applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Biochemistry
Background:
- Phosphorylated biopolymers can induce mineralization and mimic natural bone formation.
- 2-Methacryloyloxyethyl phosphorylcholine (MPC) is a biocompatible phosphorus source with limited research in bone repair.
- Chitosan-based hydrogels are explored for bone tissue engineering scaffolds.
Purpose of the Study:
- To prepare phosphorylated methacrylamide chitosan (PMAC) hydrogels.
- To investigate the mineralization ability and biocompatibility of PMAC hydrogels.
- To evaluate the potential of PMAC hydrogels for bone tissue engineering.
Main Methods:
- PMAC hydrogels were synthesized by UV irradiation of methacrylamide chitosan (MAC) and MPC.
- Characterization included assessment of mineralization ability.
- Cell culture studies with human fetal osteoblastic (hFOB) cells were performed to evaluate viability, ALP activity, and calcium deposition.
Main Results:
- PMAC hydrogels were successfully prepared with good mineralization ability.
- hFOB cells cultured on PMAC hydrogels demonstrated high viability.
- Enhanced ALP activity and calcium deposition were observed in hFOB cells on PMAC hydrogels.
Conclusions:
- PMAC hydrogels possess significant mineralization capacity.
- PMAC hydrogels support osteoblast function and proliferation.
- PMAC hydrogels show great potential as scaffolds for bone tissue engineering applications.
