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Related Experiment Video

Updated: Sep 23, 2025

Single Cell Micro-aspiration as an Alternative Strategy to Fluorescence-activated Cell Sorting for Giant Virus Mixture Separation
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Rheotaxis-based microfluidic device for selecting sperm from samples infected with a virus.

Afrouz Ataei1, Md Alamgir Kabir2, Andy W C Lau3

  • 1Department of Physics, Florida Atlantic University, Boca Raton, Florida; Asghar Lab, Micro and Nanotechnology in Medicine, College of Engineering and Computer Science, Florida Atlantic University, Boca Raton, Florida.

F&S Science
|May 13, 2022
PubMed
Summary

This study demonstrates a microfluidic device effectively separates sperm from viruses like Zika using rheotaxis. The optimized device ensures a virus-free sperm sample for assisted reproductive technology, enhancing safety and efficiency.

Keywords:
Sperm washinginfected semen samplesperm selection

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Area of Science:

  • Biomedical Engineering
  • Reproductive Biology
  • Virology

Background:

  • Semen analysis and sperm washing are crucial for assisted reproductive technology (ART).
  • Viral contamination in semen poses risks to ART procedures and patient health.
  • Existing methods for sperm washing may not always guarantee complete removal of pathogens.

Purpose of the Study:

  • To evaluate a novel rheotaxis-based microfluidic device for separating spermatozoa from viruses.
  • To assess the efficiency of the device in removing viral contaminants, specifically Zika virus, from semen samples.
  • To determine if the device can be utilized for safe and effective sperm selection and washing in ART.

Main Methods:

  • A microfluidic device exploiting the positive rheotaxis of sperm was designed and tested.
  • Human sperm samples were intentionally contaminated with viruses.
  • Experiments were conducted at different flow rates (0 and 25 μL/minute) to determine optimal washing efficiency.
  • Post-processing analysis included fluorescent microscopy and quantitative polymerase chain reaction (qPCR).

Main Results:

  • The microfluidic device successfully separated sperm from viruses at an optimal flow rate of 25 μL/minute.
  • Fluorescent microscopy and qPCR confirmed the absence of viruses in the selected sperm samples.
  • The device demonstrated efficient washing, yielding a virus-free sperm population.

Conclusions:

  • A simple, cost-effective microfluidic device was developed for sperm washing and selection.
  • The device effectively mimics the female genital tract environment.
  • This technology offers an efficient method for removing raw semen and viral contaminants during ART preparation.