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Updated: Sep 23, 2025
10:23
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
Published on: April 17, 2017
10.3K
Nuclear Lipid Droplet Birth during Replicative Stress
Sylvain Kumanski1, Romain Forey2, Chantal Cazevieille3
1Centre de Recherche en Biologie Cellulaire de Montpellier (CRBM), Université de Montpellier-Centre National de la Recherche Scientifique, 34293 Montpellier, France.
Cells
|May 14, 2022
Summary
Genotoxins causing replication stress can trigger nuclear lipid droplet (nLD) formation in yeast. This process is linked to membrane lipid composition and may impact genome stability during stress responses.
Area of Science:
- Cell Biology
- Genetics
- Molecular Biology
Background:
- The nuclear membrane safeguards genome integrity.
- Nuclear membrane deformations can compromise genetic material.
- The role of nuclear lipid droplet (nLD) formation in genome dynamics remains unclear.
Purpose of the Study:
- To investigate if genotoxins induce nLD formation.
- To determine the impact of replication stress on nLD formation.
- To explore the lipid characteristics of the nuclear membrane associated with nLD emission.
Main Methods:
- Utilized *Saccharomyces cerevisiae* as a model organism.
- Administered various genotoxins to assess nLD induction.
- Analyzed the lipid unsaturation profile and sterol content of the nuclear membrane.
Main Results:
- nLD formation is specifically triggered by genotoxins inducing replication stress.
- Exogenous and endogenous replication stress both promote nLD formation.
- Nuclear membrane phospholipid unsaturation and sterol content are linked to nLD emission.
Conclusions:
- Stressed replication forks may initiate nLD formation at the inner nuclear membrane.
- A transcriptional feedback mechanism counteracts nLD emission.
- nLDs serve as platforms for genome-modifying reactions, highlighting their role in replication stress response.
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