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Updated: Sep 23, 2025
Sentinel Lymph Node Mapping and Biopsy for Endometrial Cancer at Early Stage with Laparoscopy
Published on: August 19, 2021
Biomarkers Associated with Lymph Nodal Metastasis in Endometrioid Endometrial Carcinoma
Mathilde Mairé1, Aurélien Bourdon2, Isabelle Soubeyran3
1Department of Surgery, Institut Bergonie, 33076 Bordeaux, France.
Introduction:
Lymph node metastasis is determinant in the prognosis and treatment of endometrioid endometrial cancer (EEC) but the risk-benefit balance of surgical lymph node staging remains controversial.
Objective:
Describe the pathways associated with lymph node metastases in EEC detected by whole RNA sequencing.
Methods:
RNA-sequencing was performed on a retrospective series of 30 non-metastatic EEC. N+ and N- patients were matched for tumoral size, tumoral grade and myometrial invasion.
Results:
Twenty-eight EECs were analyzable (16 N+ and 12 N-). Bioinformatics Unsupervised analysis revealed three patterns of expression, enriched in N+, mix of N+/N- and enriched in N-, respectively. The cluster with only N+ patient overexpressed extra cellular matrix, epithelial to mesenchymal and smooth muscle contraction pathways with respect to the N- profile. Differential expression analysis between N+ and N- was used to generate a 54-genes signature with an 87% accuracy.
Conclusion:
RNA-expression analysis provides a basis to develop a gene expression-based signature that could pre-operatively predict lymph node invasion.
