Related Experiment Video

Updated: Sep 23, 2025

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants
07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

289

Oncopeptide MBOP Encoded by LINC01234 Promotes Colorectal Cancer through MAPK Signaling Pathway

Chunyuan Tang1, Ying Zhou1, Wen Sun1

  • 1Institute of Drug Metabolism and Pharmaceutical Analysis, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.

Cancers
|May 14, 2022
PubMed

Insights

Researchers discovered a new peptide, MEK1-binding oncopeptide (MBOP), derived from LINC01234. MBOP promotes colorectal cancer (CRC) growth and migration, suggesting it as a potential therapeutic target for CRC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Colorectal cancer (CRC) is a leading cause of cancer mortality globally.
  • Current diagnostic and therapeutic strategies for CRC require further advancement.
  • Noncoding RNAs (ncRNAs) and their derived peptides are emerging as significant players in biological processes.

Purpose of the Study:

  • To identify a novel ncRNA-derived peptide involved in colorectal cancer (CRC) oncogenesis.
  • To investigate the functional role and molecular mechanisms of the identified peptide in CRC.

Main Methods:

  • Bioinformatic analysis to predict coding potential of long noncoding RNAs (lncRNAs).
  • Mass spectrometry and Western blot to confirm peptide expression in CRC tissues and cell lines.
  • In vitro and in vivo assays to assess migration and proliferation.
  • Immunoprecipitation to identify interacting proteins and elucidate signaling pathways.

Main Results:

  • An 85-amino-acid peptide, MEK1-binding oncopeptide (MBOP), encoded by LINC01234, was identified.
  • MBOP is overexpressed in CRC tissues and cell lines.
  • MBOP promotes CRC cell migration and proliferation.
  • MBOP interacts with MEK1, activating the MEK1/pERK/MMP2/MMP9 signaling axis.
  • MBOP degradation is mediated by E3 ligases MAEA and RMND5A.

Conclusions:

  • MBOP functions as an oncogenic peptide in colorectal cancer.
  • MBOP may serve as a potential prognostic biomarker for CRC.
  • MBOP represents a potential therapeutic target for CRC treatment.
Keywords:
LncRNAMAPK signaling pathwayMBOPcolorectal cancerpeptide

More Related Videos

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer06:51

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Published on: July 21, 2018

18.1K
Zebrafish Model of Neuroblastoma Metastasis05:20

Zebrafish Model of Neuroblastoma Metastasis

Published on: March 14, 2021

2.9K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.9K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
6.3K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.6K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
4.4K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.5K