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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Sep 23, 2025

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Published on: May 5, 2017

The evolution of spectral flow cytometry.

¹Scintillon Institute, San Diego, California, USA.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology

|May 14, 2022

Summary

This summary is machine-generated.

Spectral flow cytometry has evolved from an emerging technology to a transformative force in cytometry and single-cell analysis. This review highlights its origins, current state, and future potential in biomedical research.

Keywords:

detector array dispersive optics excitation-emission matrix unmixing

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Last Updated: Sep 23, 2025

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Area of Science:

Biotechnology
Cellular Biology
Analytical Chemistry

Background:

Spectral flow cytometry has transitioned from niche research to a mainstream technology.
Its development is driven by the need for multiparameter immunophenotyping in biomedical research.
Current instrumentation and software primarily cater to immune cell analysis.

Purpose of the Study:

To review the origins and milestones of spectral flow cytometry.
To assess the current state of spectral flow cytometry instrumentation and software.
To speculate on future directions and applications of this technology.

Main Methods:

Historical review of spectral flow cytometry development.
Assessment of current instrumentation and software capabilities.
Analysis of emerging applications and future trends.

Main Results:

Spectral flow cytometry is now a transformative force in cytometry and single-cell analysis.
The technology's potential, particularly in high-resolution spectral property analysis, is beginning to be realized.
Significant advancements have been made to meet the demands of modern biomedical research.

Conclusions:

Spectral flow cytometry is poised to significantly shape the future of cytometry and single-cell analysis.
Further exploitation of high-resolution spectral analysis promises expanded applications.
Continued innovation in instrumentation and software will drive future advancements.