Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pasteurization and Food Preservation01:28

Pasteurization and Food Preservation

Pasteurization is a widely employed thermal processing technique designed to enhance the safety and shelf life of perishable food and beverages. By subjecting products to specific high temperatures for controlled durations, this method effectively inactivates pathogenic microorganisms and spoilage enzymes without significantly compromising sensory qualities. The technique has been pivotal in food safety management, especially for consumables susceptible to microbial contamination such as milk,...
Principles of Food Preservation01:27

Principles of Food Preservation

Food spoilage results from microbial growth, enzymatic activity, and environmental factors that gradually degrade the sensory, nutritional, and safety qualities of food. Preservation techniques aim to slow or halt these processes to extend shelf life and maintain product quality.A key concept in food microbiology is the microbial growth curve, which includes four phases: lag, exponential (log), stationary, and death. During the lag phase, bacteria adjust to their environment without significant...
Antiviral Nucleoside Inhibitors01:22

Antiviral Nucleoside Inhibitors

Antiviral Nucleoside InhibitorsAntiviral nucleoside inhibitors are structural analogs of natural nucleosides that interfere with viral DNA or RNA synthesis. These compounds selectively target viral polymerases due to their resemblance to host nucleosides, thereby disrupting viral genome replication.Mechanism of Acyclovir ActionAcyclovir is a guanosine analog with a three-carbon acyclic side chain. It selectively targets herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2),...
Inhibitors of Virion Maturation and Assembly01:19

Inhibitors of Virion Maturation and Assembly

As part of their replication cycle, certain viruses synthesize long precursor proteins called polyproteins within infected host cells. In human immunodeficiency virus (HIV), two major polyproteins are produced: Gag and Gag-Pol. The Gag polyprotein supplies the structural components of the virus, while Gag-Pol includes essential viral enzymes such as reverse transcriptase, integrase, and protease. After synthesis, these polyproteins move to the host cell membrane, where they assemble into an...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preparationof Konjac Glucomannan-Based Composite Films Loaded with Hydroxypropyl-β-Cyclodextrin/Hesperetin Inclusion Complex and Their Preservation Effect on Strawberries.

Foods (Basel, Switzerland)·2026
Same author

Hemorrhoids or not? Inflammatory cloacogenic polyps mimicking prolapsed hemorrhoids: a 10-year case series of 56 patients.

Revista espanola de enfermedades digestivas·2026
Same author

Interfacial Charge Separation Engineering-Modulated Multichannel Photoelectrochemical Biosensor Enabled by a Triple-Responsive Nanolabel.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Urolithin A Mitigates Renal Fibrosis by Promoting Fatty Acid Oxidation Through Orchestrating β-Catenin Signaling.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

Spatiotemporal Distribution Characteristics and Driving Factors of Phytoplankton in the Mainstream of the Yellow River (Shandong Section).

Microorganisms·2026
Same author

A study of 25-(OH)D<sub>3</sub> levels in children with type 1 diabetes mellitus and its influencing factors.

Experimental and therapeutic medicine·2026

Related Experiment Video

Updated: Jul 17, 2026

Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis
09:35

Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis

Published on: August 9, 2013

11.8K

Ascorbic acid addition during dehydration improves garlic shoot tip cryopreservation but does not affect viral load.

Xiaodong Xing1, Min Liu1, Rong Zhou1

  • 1College of Horticulture, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in East China, Ministry of Agriculture, Nanjing, China.

Cryobiology
|May 14, 2022
PubMed
Summary

Adding ascorbic acid (AsA) during the dehydration step of cryopreservation significantly improves garlic survival by reducing oxidative damage. This method enhances plant regrowth without impacting virus elimination efficiency.

Keywords:
Ascorbic acidCryopreservationGarlicOxidative damageShoot tips

More Related Videos

Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy
13:35

Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy

Published on: May 5, 2017

11.0K
Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity
07:32

Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity

Published on: April 13, 2021

3.3K

Related Experiment Videos

Last Updated: Jul 17, 2026

Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis
09:35

Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis

Published on: August 9, 2013

11.8K
Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy
13:35

Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy

Published on: May 5, 2017

11.0K
Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity
07:32

Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity

Published on: April 13, 2021

3.3K

Area of Science:

  • Plant science
  • Cryobiology
  • Biotechnology

Background:

  • Cryopreservation is effective for virus elimination in garlic but causes oxidative damage, impacting survival.
  • Ascorbic acid (AsA) can mitigate oxidative stress and improve post-cryopreservation recovery.
  • The optimal timing for AsA application during cryopreservation requires investigation.

Purpose of the Study:

  • To determine the optimal step for adding ascorbic acid (AsA) during garlic cryopreservation to minimize oxidative damage and enhance survival.
  • To investigate the effects of AsA on reactive oxygen species (ROS) accumulation and antioxidant enzyme activity.
  • To correlate cell viability with ROS levels, lipid peroxidation, and antioxidant indicators.

Main Methods:

  • Garlic shoot tips were cryopreserved with AsA added during osmoprotection (O) or dehydration (DE) steps.
  • Cell viability, ROS levels, and membrane lipid peroxidation were monitored dynamically.
  • Gene expression of antioxidant enzymes was analyzed.
  • Correlation analyses were performed between various physiological indicators.

Main Results:

  • Ascorbic acid significantly reduced ROS accumulation in garlic shoot tips.
  • Increased gene expression of antioxidant enzymes was observed with AsA treatment.
  • Membrane lipid peroxidation, driven by ROS, was identified as the primary factor affecting cell viability.
  • Adding AsA during the dehydration step maximally reduced ROS and alleviated oxidative damage, significantly improving garlic survival and regrowth.

Conclusions:

  • The dehydration step is optimal for AsA application during garlic cryopreservation to enhance survival and regrowth.
  • Ascorbic acid effectively scavenges ROS and mitigates oxidative stress, thereby improving cryopreservation outcomes.
  • While AsA improves plant survival, it did not significantly alter virus elimination rates in regenerated garlic plants.