Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.6K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.6K
Protein-protein Interfaces02:04

Protein-protein Interfaces

13.8K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
13.8K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.3K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.3K
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

2.6K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.6K
Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

8.8K
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
8.8K
Protein Modifications in the RER01:26

Protein Modifications in the RER

5.7K
Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
5.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell-Free In Vitro mRNA Translation In Drosophila Lysates.

Journal of visualized experiments : JoVE·2026
Same author

Translational regulation of human papillomavirus mRNAs in carcinogenesis: old questions and new insights.

Frontiers in cell and developmental biology·2025
Same author

Deep Sequencing Reveals Novel Mutations in <i>Androgen Receptor</i>-Related Genes in Prostate Cancer.

International journal of molecular sciences·2025
Same author

The Bright Future of mRNA as a Therapeutic Molecule.

Genes·2025
Same author

AUGcontext DB: a comprehensive catalog of the mRNA AUG initiator codon context across eukaryotes.

RNA biology·2025
Same author

Analysis of eIF4E-family members in fungi contributes to their classification in eukaryotes.

The Journal of biological chemistry·2024

Related Experiment Video

Updated: Sep 23, 2025

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
08:47

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

3.2K

The versatile relationships between eIF4E and eIF4E-interacting proteins.

Greco Hernández1

  • 1Translation and Cancer Laboratory, Unit of Biomedical Research on Cancer, National Institute of Cancer (Instituto Nacional de Cancerología, INCan). 22 San Fernando Avenue, Tlalpan, 14080-Mexico City, Mexico.

Trends in Genetics : TIG
|May 14, 2022
PubMed
Summary
This summary is machine-generated.

The eukaryotic initiation factor 4E (eIF4E) is vital for RNA metabolism and gene expression. It interacts with numerous proteins to regulate key cellular processes, highlighting its central role.

Keywords:
P bodieseIF4EeIF4E-interacting proteinsmRNA storagemRNA transporttranslation initiation

More Related Videos

Identification of Protein Interacting Partners Using Tandem Affinity Purification
10:02

Identification of Protein Interacting Partners Using Tandem Affinity Purification

Published on: February 25, 2012

37.7K
Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
12:53

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics

Published on: July 6, 2014

31.6K

Related Experiment Videos

Last Updated: Sep 23, 2025

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
08:47

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

3.2K
Identification of Protein Interacting Partners Using Tandem Affinity Purification
10:02

Identification of Protein Interacting Partners Using Tandem Affinity Purification

Published on: February 25, 2012

37.7K
Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
12:53

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics

Published on: July 6, 2014

31.6K

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Gene Expression

Background:

  • RNA metabolism and gene expression are fundamental to cellular functions.
  • The eukaryotic initiation factor 4E (eIF4E) is a key regulator in these processes.
  • eIF4E's function is dependent on its interactions with a diverse set of proteins.

Purpose of the Study:

  • To elucidate the central role of eIF4E in RNA metabolism and gene expression.
  • To highlight the importance of protein-protein interactions for eIF4E function.
  • To underscore eIF4E's critical involvement in mRNA processing, transport, translation, and storage.

Main Methods:

  • Literature review of studies on eIF4E.
  • Analysis of protein-protein interaction networks involving eIF4E.
  • Functional studies on mRNA fate regulated by eIF4E.

Main Results:

  • eIF4E acts as a crucial hub connecting RNA metabolism and gene expression.
  • Complex formation with various proteins enables eIF4E's diverse functions.
  • eIF4E critically influences mRNA processing, transport, translation, and storage.

Conclusions:

  • eIF4E is indispensable for cellular life, mediating core processes of RNA metabolism and gene expression.
  • The intricate network of eIF4E-interacting proteins is essential for its biological activities.
  • Understanding eIF4E's interactions provides insights into cellular regulation.