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Updated: Sep 23, 2025

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Published on: October 10, 2013

Simultaneous quantification of specific food allergen proteins using a fluorescent multiplex array.

Stephanie C Filep¹, Kristina Reid Black¹, Bryan R E Smith¹

¹InBio, Food Science Group, Charlottesville, VA 22903, USA.

|May 15, 2022

Summary

This summary is machine-generated.

This study developed a fluorescent multiplex array for detecting 10 regulated food allergens simultaneously. This efficient tool aids in food allergen risk assessment and therapeutic product standardization.

Keywords:

Allergen testing Allergens Food allergy Food regulations Multiplex array

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Area of Science:

Food Science and Technology
Analytical Chemistry
Immunology

Background:

Accurate quantification of food allergens is crucial for consumer safety and regulatory compliance.
Existing methods for food allergen detection can be time-consuming and may not cover a broad range of allergens simultaneously.
The need for efficient, simultaneous detection of multiple regulated food allergens is increasing.

Purpose of the Study:

To develop and validate a fluorescent multiplex array for the simultaneous measurement of regulated food allergens.
To assess the array's performance using specific allergen protein molecules and reference materials.
To demonstrate the array's applicability in analyzing incurred allergens in complex food matrices.

Main Methods:

Development of a fluorescent multiplex array utilizing microspheres coupled to specific monoclonal antibodies.
Detection of 17 allergens across 10 food categories including peanut, tree nut, milk, egg, soy, fish, shellfish, sesame, mustard, and celery.
Validation using purified allergens as reference standards, establishing standard curves over a 5-log dynamic range and assessing assay performance criteria (70-130% recovery, ≤15% CV).

Main Results:

The multiplex array demonstrated reliable quantification of 17 allergens with established dynamic range and acceptable intra- and inter-assay variability.
Analysis of food reference materials confirmed high levels of major allergens (2000-175,000 µg/g).
Detection of specific allergens (egg, milk, peanut, hazelnut, walnut) in chocolate bars at low parts-per-million (ppm) levels (3-100 ppm) with incurred allergens.

Conclusions:

The developed fluorescent multiplex array is an efficient and sensitive tool for the simultaneous measurement of multiple regulated food allergens.
The array shows significant potential for applications in food safety risk assessment.
This method can contribute to the standardization of therapeutic products for food allergies.