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Ultrasensitive aptasensor for arsenic detection using quantum dots and guanylated Poly(methacrylamide).

Gurpreet K Soni¹, Nishima Wangoo², Ceren Cokca³

¹Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University, Sector-14, Chandigarh, 160014, India.

Analytica Chimica Acta

|May 15, 2022

Summary

A novel aptasensor using poly (HPMA-s-GPMA) copolymer and quantum dots detects arsenite (As³⁺) without labels. This method offers sensitive, selective, and rapid arsenite detection in various samples.

Keywords:

Aptasensor Arsenic detection Fluorometric Guanylated poly(methacrylamide)Picomolar detection Quantum dots

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Area of Science:

* Materials Science
* Analytical Chemistry
* Environmental Science

Background:

* Arsenite (As³⁺) poses significant health risks, necessitating sensitive detection methods.
* Existing arsenite detection techniques often lack selectivity, speed, or require labels.
* Quantum dots (QDs) offer unique optical properties for sensing applications.

Purpose of the Study:

* To develop a novel, label-free aptasensor for the detection of arsenite (As³⁺).
* To utilize a three-component system involving a novel copolymer, QDs, and an aptamer for arsenite sensing.
* To achieve simple, selective, and rapid detection of arsenite in various real-world samples.

Main Methods:

* Fabrication of a three-component aptasensor using guanidinium-bearing poly (HPMA-s-GPMA) copolymer and MPA-CdTe@CdS quantum dots (QDs).
* Exploitation of a fluorescence quenching mechanism based on competitive binding between arsenite, copolymer, and aptamer.
* Utilized As³⁺-specific aptamer for selective binding and electrostatic interactions with QDs and copolymer.

Main Results:

* The aptasensor demonstrated a limit of detection (LOD) of 246.77 pM for arsenite within the 0.01-100 nM range.
* Achieved excellent specificity for As³⁺ ions, even in the presence of interfering metal ions.
* Successfully applied the sensing platform to real-world samples including water, food, and soil.

Conclusions:

* The developed poly (HPMA-s-GPMA) copolymer-based aptasensor provides a sensitive and selective method for arsenite detection.
* The label-free, fluorescence quenching approach offers a cost-effective and rapid sensing platform.
* This study paves the way for developing advanced devices for on-site arsenic detection.