Thyroid hormones in blood plasma of developing salmon embryos

Blood plasma thyroxine (T4) and 3,5,3'-triiodo-L-thyronine (T3) concentrations in developing embryos of chum (Oncorhynchus keta), coho (Oncorhynchus kisutch), chinook (Oncorhynchus tschawytscha), and Atlantic salmon (Salmo salar) were measured by radioimmunoassay between hatching and completion of yolk-sac absorption. Blood sampling was initiated when approximately 50% of the yolk that was present at hatching had been absorbed. At this time blood plasma levels of T4 (7-9 ng/ml) and T3 (1-5 ng/ml) were similar in all species. In embryos of each salmon species blood plasma levels of T4 increased to maximal values (9-16 ng/ml) when both differentiation of the abdominal body wall and absorption of the yolk sac were completed. Blood plasma levels of T3 generally decreased to low or nondetectable values during this time. There was either a trend toward a decrease or a stasis of levels of T4 and at the same time in some species an increase in levels of T3 in the blood plasma following the completion of yolk-sac absorption and the onset of exogenous feeding. These results demonstrate that thyroid hormones are present in the blood of developing teleost embryos. This finding is discussed in light of the possible role of thyroid hormones in control of the embryonic and larval development of fishes.