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An Automated Quantification Tool for Angiogenic Sprouting From Endothelial Spheroids.

Pavitra Kannan1, Martin Schain2, David P Lane1

  • 1Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.

Frontiers in Pharmacology
|May 16, 2022
PubMed
Summary

We developed AQuTAS, an automated tool for analyzing sprouting angiogenesis in vitro. This reproducible and sensitive method accurately quantifies sprouting parameters, improving upon manual analysis.

Keywords:
automated quantificationin vitro angiogenesis assayspheroidssprouting angiogenesissprouting parameters

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Area of Science:

  • * Cell biology
  • * Angiogenesis research
  • * Bio-imaging analysis

Background:

  • * Sprouting angiogenesis is crucial for development and disease.
  • * In vitro sprouting assays like the spheroid-based assay are common.
  • * Manual analysis of these assays is time-consuming and lacks reproducibility.

Purpose of the Study:

  • * To develop an automated analysis tool (AQuTAS) for spheroid-based sprouting assays.
  • * To quantify sprouting parameters in a reproducible and sensitive manner.
  • * To overcome limitations of manual analysis in angiogenesis research.

Main Methods:

  • * Development and validation of the AQuTAS automated analysis tool.
  • * Training and testing the algorithm on diverse datasets.
  • * Sensitivity testing using pro- and antiangiogenic compounds.

Main Results:

  • * AQuTAS accurately quantifies sprouting parameters from spheroid-based assays.
  • * The tool detects known differences induced by pro- and antiangiogenic compounds.
  • * Cumulative sprout length demonstrated the highest effect size for distinguishing sprouting behavior.

Conclusions:

  • * AQuTAS provides a reproducible and sensitive automated method for analyzing sprouting angiogenesis.
  • * The tool enhances the efficiency and reliability of in vitro angiogenesis studies.
  • * AQuTAS facilitates the discovery of novel pro- and antiangiogenic factors.