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Size Distribution of Microparticles: A New Parameter to Predict Acute Lung Injury After Cardiac Surgery With
Hao-Xiang Yuan1,2,3,4, Kai-Feng Liang1,2,3,4, Chao Chen1,2,3,4
1Division of Cardiac Surgery, Heart Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Background:
Acute lung injury (ALI) is a common complication after cardiac surgery with cardiopulmonary bypass (CPB). No precise way, however, is currently available to predict its occurrence. We and others have demonstrated that microparticles (MPs) can induce ALI and were increased in patients with ALI. However, whether MPs can be used to predict ALI after cardiac surgery with CPB remains unknown.
Methods:
In this prospective study, 103 patients undergoing cardiac surgery with CPB and 53 healthy subjects were enrolled. MPs were isolated from the plasma before, 12 h after, and 3 d after surgery. The size distributions of MPs were measured by the LitesizerTM 500 Particle Analyzer. The patients were divided into two subgroups (ALI and non-ALI) according to the diagnosis of ALI. Descriptive and correlational analyzes were conducted between the size distribution of MPs and clinical data.
Results:
Compared to the non-ALI group, the size at peak and interquartile range (IQR) of MPs in patients with ALI were smaller, but the peak intensity of MPs is higher. Multivariate logistic regression analysis indicated that the size at peak of MPs at postoperative 12 h was an independent risk factor for ALI. The area under the curve (AUC) of peak diameter at postoperative 12 h was 0.803. The best cutoff value of peak diameter to diagnose ALI was 223.05 nm with a sensitivity of 88.0% and a negative predictive value of 94.5%. The AUC of IQR at postoperative 12 h was 0.717. The best cutoff value of IQR to diagnose ALI was 132.65 nm with a sensitivity of 88.0% and a negative predictive value of 92.5%. Combining these two parameters, the sensitivity reached 92% and the negative predictive value was 96%.
Conclusions:
Our findings suggested that the size distribution of MPs could be a novel biomarker to predict and exclude ALI after cardiac surgery with CPB.
Insights
Microparticle (MP) size distribution can predict acute lung injury (ALI) after cardiac surgery. Smaller MP size at peak and higher intensity at 12 hours post-surgery indicate increased ALI risk.
Area of Science:
- Cardiovascular Surgery
- Pulmonary Medicine
- Biomarker Discovery
Background:
- Acute lung injury (ALI) is a frequent complication following cardiac surgery with cardiopulmonary bypass (CPB).
- Current methods for predicting ALI post-CPB are imprecise.
- Microparticles (MPs) are implicated in ALI development and elevated in ALI patients, but their predictive value remains unestablished.
Purpose of the Study:
- To investigate if microparticle (MP) size distribution can serve as a predictive biomarker for acute lung injury (ALI) after cardiac surgery with cardiopulmonary bypass (CPB).
Main Methods:
- Prospective study enrolling 103 cardiac surgery patients and 53 healthy controls.
- Isolation and size distribution analysis of plasma microparticles (MPs) at pre-surgery, 12 hours post-surgery, and 3 days post-surgery.
- Comparison of MP size distribution between ALI and non-ALI patient subgroups.
Main Results:
- Patients with ALI exhibited smaller peak size and interquartile range (IQR) but higher peak intensity of MPs compared to non-ALI patients.
- Postoperative 12-hour peak MP size (AUC 0.803) and IQR (AUC 0.717) were significant predictors of ALI.
- Combining peak size and IQR achieved 92% sensitivity and 96% negative predictive value for ALI detection.
Conclusions:
- Microparticle (MP) size distribution represents a novel biomarker for predicting and excluding acute lung injury (ALI) post-cardiac surgery with CPB.
- Specific MP size parameters offer high diagnostic accuracy for ALI.
