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Updated: Sep 23, 2025
Spontaneous Murine Model of Anaplastic Thyroid Cancer
Published on: February 3, 2023
Expression of colorectal neoplasia differentially expressed in anaplastic thyroid carcinoma and its effect on cancer
Lili Du1, Qingsong Zhao2, Jingjing Li1
1Department of Endocrinology and Metabolism, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Background:
The incidence of anaplastic thyroid cancer (ATC) is high among human cancers. Colorectal neoplasia differentially expressed (CRNDE) is highly expressed in common tumors, and is therefore a potential molecular target for anti-tumor therapy. However, the function of CRNDE in ATC remains elusive.
Methods:
The Gene Expression Omnibus (GEO) database was used to screen the differential expression of long-noncoding RNA (lncRNA) in ATC tissues. The Cancer Genome Atlas (TCGA) database was used to analyze the expression of CRNDE in thyroid cancer (THCA) tissues and its impact on patient prognosis. Quantitative real-time PCR (qRT-PCR) was used to determine the expression level of CRNDE in tumor and control tissues. The biological function of CRNDE in THCA was explored using TCGA RNA sequencing (RNA-seq) data analysis. ATC cell lines with low and high CRNDE expression were selected for CRNDE siRNA transfection, and the proliferation of cells was detected in each group.
Results:
The GEO and TCGA databases analysis results showed that CRNDE was highly expressed in ATC tissues, which is related to the poor prognosis of THCA patients. Also, the expression of CRNDE in the ATC cell line, ARO (human thyroid cancer cell line), was relatively high, while the expression in sw579 is relatively low. Therefore, ARO and sw579 were chosen for CRNDE small interfering RNA (siRNA) transfection. Compared with negative control (si-NC), the expression of CRNDE in si-CRNDE-1, si-CRNDE-2, and si-CRNDE-3 was reduced, indicating that the inhibitory effect was significantly enhanced and the cell proliferation ability was reduced, and the cell cycle is arrested in the G0/G1 phase. Finally, it was found that the wnt3a, β-catenin, and cyclinD1 protein expressions of si-CRNDE-1 and si-CRNDE-2 were significantly reduced.
Conclusions:
The high expression of CRNDE in ATC tissues may promote the proliferation of ATC cells by regulating the Wnt/β-catenin signaling pathway. CRNDE may be a potential molecular target for the treatment of ATC.
Insights
Colorectal neoplasia differentially expressed (CRNDE) is highly expressed in anaplastic thyroid cancer (ATC), promoting cell proliferation. Targeting CRNDE may offer a new therapeutic strategy for ATC treatment.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Anaplastic thyroid cancer (ATC) has a high incidence among human cancers.
- Colorectal neoplasia differentially expressed (CRNDE) is upregulated in various tumors, suggesting its potential as a therapeutic target.
- The specific role of CRNDE in ATC remains largely unexplored.
Purpose of the Study:
- To investigate the expression and function of CRNDE in anaplastic thyroid cancer.
- To determine the impact of CRNDE on thyroid cancer patient prognosis.
- To explore the potential of CRNDE as a molecular target for ATC therapy.
Main Methods:
- Utilized Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases to analyze CRNDE expression in thyroid cancer (THCA).
- Employed quantitative real-time PCR (qRT-PCR) to validate CRNDE expression in tumor and control tissues.
- Conducted siRNA-mediated knockdown of CRNDE in ATC cell lines and analyzed cell proliferation, cell cycle, and key protein expressions.
Main Results:
- CRNDE was found to be highly expressed in ATC tissues and associated with poor prognosis in THCA patients.
- CRNDE knockdown in ATC cell lines (ARO) significantly reduced cell proliferation and induced G0/G1 cell cycle arrest.
- CRNDE inhibition led to decreased expression of Wnt3a, β-catenin, and cyclin D1 proteins.
Conclusions:
- High CRNDE expression in ATC may drive tumor cell proliferation via the Wnt/β-catenin signaling pathway.
- CRNDE represents a promising molecular target for the development of novel anti-cancer therapies for ATC.
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